Host response of human epidermis to Methicillin-resistant Staphylococcus aureus biofilm infection and synthetic antibiofilm peptide treatment
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ABSTRACT:
PROVIDER: PRJNA866534 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR20862383.fastq.gz | Fastqsanger.gz | |||
| SRR20862384.fastq.gz | Fastqsanger.gz | |||
| SRR20862385.fastq.gz | Fastqsanger.gz | |||
| SRR20862386.fastq.gz | Fastqsanger.gz | |||
| SRR20862387.fastq.gz | Fastqsanger.gz |
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Project description:Bacterial biofilm infections associated with wounded skin are prevalent, recalcitrant, and in urgent need of treatments. Additionally, host responses in the skin to biofilm infections are not well understood. Here we employed a human organoid skin model to explore the transcriptomic changes of thermally-injured epidermis to methicillin-resistant Staphylococcus aureus (MRSA) biofilm colonization. MRSA biofilm impaired skin barrier function, enhanced extracellular matrix remodelling, elicited inflammatory responses including IL-17, IL-12 family and IL-6 family interleukin signalling, and modulated skin metabolism. Synthetic antibiofilm peptide DJK-5 effectively diminished MRSA biofilm and associated skin inflammation in wounded human ex vivo skin. In the epidermis, DJK-5 shifted the overall skin transcriptome towards homeostasis including modulating the biofilm induced inflammatory response, promoting the skin DNA repair function, and downregulating MRSA invasion of thermally damaged skin. These data clarified the underlying immunopathogenesis of biofilm infections and revealed the intrinsic promise of synthetic peptides in reducing inflammation and biofilm infections.
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