Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [AR_SNR_RNAseq]

ABSTRACT: Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [AR_SNR_RNAseq]

PROVIDER: PRJNA867158 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [CRISPRscreen]

Project description:The ability of Mycobacterium tuberculosis (Mtb) to establish latency directly impacts disease and response to treatment but the host’s influence on this switch remains elusive. Using a novel Mtb reporter strain to separate macrophages infected with replicating or latent Mtb, we performed transcriptomics and genome-wide CRISPR screens. After validating the recovery of known susceptibility loci from TB GWAS as modifiers of active replication, we identified multiple pathways and novel modulators of latency. We validated multiple controllers of latency and mechanistically characterized the transporter MMGT1. MMGT1-deficient macrophages upregulated lipid metabolism genes and accumulated lipid droplets during infection. Critically, targeting triacylglycerol synthesis reduced both droplet formation and Mtb latency. We identified GPR156, an orphan GPCR as a key inducer of droplet accumulation in ΔMMGT1 cells. Altogether, our work uncovers the role of MMGT1-GPR156-lipid droplets in the induction of Mtb latency, suggesting that host-directed therapies could be used to modulate Mtb phenotypes during infection.

2023-06-01 | GSE210689 | GEO

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [AR_SNR_RNAseq]

2023-06-01 | GSE210687 | GEO

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [THP1_MMGT1_RNAseq]

2023-06-01 | GSE210690 | GEO

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence

Project description:Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence

| PRJNA867146 | ENA

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [THP1_MMGT1_RNAseq]

Project description:Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [THP1_MMGT1_RNAseq]

| PRJNA867155 | ENA

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence [CRISPRscreen]

| PRJNA867156 | ENA

Identification of host regulators of Mycobacterium tuberculosis phenotypic states uncovers a role for the MMGT1-GPR156-lipid droplet axis in the induction of persistence

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-06-01 | GSE210691 | GEO

A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease states

Project description:A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease states

| PRJNA738478 | ENA

Transcriptomic profiling uncovers key regulators during tulip bulb development

Project description:Transcriptomic profiling uncovers key regulators during tulip bulb development

| PRJNA704900 | ENA