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Phosphate depletion in mammalian cells

ABSTRACT: Phosphate depletion in mammalian cells

PROVIDER: PRJNA867397 | ENA |

REPOSITORIES: ENA

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CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters

Project description:This SuperSeries is composed of the following subset Series: GSE38560: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (RNA-seq) GSE38561: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (ChIP-seq) GSE38562: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (genomic SEQ) GSE38563: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (MNase-seq) GSE38564: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (5) Refer to individual Series

2012-06-11 | E-GEOD-38577 | biostudies-arrayexpress

Sequencing of yeast mutants with or without phosphate depletion

Project description:Sequencing of yeast mutants with or without phosphate depletion

| PRJNA877262 | ENA

Treated Blood Cells, Cyclophosphamide, Fludarabine Phosphate, and Aldesleukin in Treating Patients With Cancer

Project description:RATIONALE: Placing a gene into T cells may improve the body’s ability to recognize cancer cells and build an immune response to fight cancer. Drugs used in chemotherapy, such as cyclophosphamide and fludarabine phosphate, work in different ways to stop the growth of tumor cells, either by killing the cells or by stopping them from dividing. Biological therapies, such as aldesleukin, may stimulate the immune system in different ways and stop cancer cells from growing. Giving specially treated T cells together with cyclophosphamide, fludarabine phosphate, and aldesleukin may kill more tumor cells. PURPOSE: This phase I clinical trial is studying the side effects and best dose of treated T cells when given together with cyclophosphamide, fludarabine phosphate, and aldesleukin in treating patients with cancer.

| 2095938 | ecrin-mdr-crc

Phosphate depletion in mammalian cells

Project description:Comparison of different cell lines membrane potential responded or not to -Pi (HEK293 and A375 has increased mp in -Pi, but not U2OS)

2025-07-15 | GSE210769 | GEO

WDR26 depletion alters chromatin accessibility and gene expression profiles in mammalian cells [RNA-seq]

Project description:WDR26 depletion alters chromatin accessibility and gene expression profiles in mammalian cells [RNA-seq]

| PRJNA1139852 | ENA

WDR26 depletion alters chromatin accessibility and gene expression profiles in mammalian cells [ATAC-seq]

Project description:WDR26 depletion alters chromatin accessibility and gene expression profiles in mammalian cells [ATAC-seq]

| PRJNA1139849 | ENA

Phosphate depletion: A novel trigger for Mycobacterium tuberculosis persistence

Project description:These data represent the global gene expression profile of Mycobacterium tuberculosis after 24 hrs and 72 hrs of inorganic phosphate starvation. Differentially regulated genes appear to include those encoding proteins involved in adaptation to phosphate starvation, namely those involved in phosphate regulation and phosphate assimilation, as well as those involved in the stringent response.

2009-09-22 | GSE14840 | GEO

CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (ChIP-seq)

Project description:One clear hallmark of mammalian promoters is the presence of CpG islands (CGIs) at more than two thirds of genes whereas TATA boxes are only present at a minority of promoters. Using genome-wide approaches, we show that GC content and CGIs are major promoter elements in mammalian cells, able to govern open chromatin conformation and support paused transcription. First, we define three classes of promoters with distinct transcriptional directionality and pausing properties which correlate with their GC content. We further analyze the direct influence of GC content on nucleosome positioning and depletion, and show that CGIs correlate with nucleosome depletion both in vivo and in vitro. We also show that transcription is not essential for nucleosome exclusion but influences both a weak +1 and a well-positioned nucleosome at CGI borders. Altogether our data support the idea that CGIs have become an essential feature of promoter structure defining novel regulatory properties in mammals. Nucleosome density and positioning were studied by high-throughput sequencing of DNA previously treated with Mnase. In parallel, chIPseq for PolII and H3K27ac were performed in mouse and human with different conditions to assess a potential effect of transcription on nucleosomes properties.

2012-06-12 | E-GEOD-38561 | biostudies-arrayexpress

CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (MNase-seq)

2012-06-12 | E-GEOD-38563 | biostudies-arrayexpress

Kidney gene expression in response to high phosphate diet

Project description:Phosphate homeostasis is a known critical function performed by the mammalian kidneys. However, changes to the transcriptome of the mammalian kidney in response to phosphate loading is unclear. The goal of this study was to examine kidney gene expression in response to high phosphate diet, compared to normal phosphate diet. RNA-seq was performed on the kidneys of C57BL/6J mice who were fed a high phosphate diet and in mice who received a normal phosphate diet for three days.

2023-03-20 | GSE223176 | GEO

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