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Transcriptional profiling of striatum in METH self-administered rats

ABSTRACT: Transcriptional profiling of striatum in METH self-administered rats

PROVIDER: PRJNA869440 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21043184_1.fastq.gz	Fastqsanger.gz
	SRR21043184_2.fastq.gz	Fastqsanger.gz
	SRR21043185_1.fastq.gz	Fastqsanger.gz
	SRR21043185_2.fastq.gz	Fastqsanger.gz
	SRR21043186_1.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of striatum in METH self-administered rats [miRNA]

Project description:This study aimed to identify changes in novel miRNAs and their target genes during METH self-administration and investigate their roles in METH-induced locomotion. RNA sequencing analysis revealed that mir-183-5p was upregulated in the striatum of METH self-administered rats, and target gene prediction revealed that the glucocorticoid receptor (GR) gene, Nr3c1, was a potential target gene for mir-183-5p. We confirmed that single and repeated METH administrations increased METH-induced locomotion and plasma corticosterone levels in rats.

2022-10-19 | GSE211185 | GEO

Transcriptional profiling of striatum in METH self-administered rats [mRNA]

2022-10-19 | GSE211184 | GEO

Transcriptional profiling of striatum in METH self-administered rats [mRNA]

Project description:Transcriptional profiling of striatum in METH self-administered rats [mRNA]

| PRJNA869444 | ENA

Transcriptional profiling of the striatum from METH self-administered rats, including those after 12 and 24 hours of abstinence.

Project description:This study aimed to identify transcriptome markers for diagnosis in each phase of METH self-administration and abstinence after METH self-administration, and to investigate the role of these markers. In addition, we aimed to identify transcriptomes involved in resistance to drug-seeking behavior in a group that did not show drug-seeking behavior after METH self-administration, and to elucidate their roles. We identified an impressive transcriptomes in the striatum of rats during repeated METH administration and abstinence phases.

2025-12-03 | GSE283226 | GEO

RNA sequencing analysis identifies sex differences in transcriptional signatures in the dorsal striatum of female and male rats after withdrawal from methamphetamine self-administration

Project description:Significant methamphetamine (METH)-induced behavioral differences exist between the two sexes of humans and other animals. These dissimilarities may be related to sexual dimorphism in baseline molecular and biochemical mechanisms in brain reward neuroanatomical pathways. As a first step towards identifying sex-based differences in methamphetamine-induced transcriptional signatures, we used RNA sequencing analysis to measure genome-wide changes in gene expression in the dorsal striatum of rats that had self-administered METH. We trained rats to self-administer METH (0.1 mg/kg/infusion, i.v.) using two 3-hr daily sessions (with 30 minutes time out between session) for 20 days. Control rats self-administered saline under similar conditions. This was followed by drug seeking tests on withdrawal days 3 (WD3) and 30 (WD30). Behavioral results show that male rats took more METH than female rats. In both male and female rats, some animals escalated (high-takers) whereas others did not escalate (low-takers) their METH intake during the behavioral experiment. Rats were euthanized 24 hours after the second drug seeking test. RNA was extracted from the dorsal striatum (dSTR) and used in RNA sequencing analysis. The data identified substantial baseline differences in gene expression between female and male control rats. In addition, METH use and withdrawal were associated with significant sex-related differences in changes in striatal gene expression, with minimal overlaps of altered mRNAs. Thus, the present results provide further supporting evidence for sexually dimorphic responses to METH exposure. These observations support the notion of sex-specific approaches to the treatment of patients who suffer from METH use disorder.

2025-06-16 | GSE280589 | GEO

Transcriptional profiling of the striatum from METH self-administered rats, including those after 12 and 24 hours of abstinence.

Project description:Transcriptional profiling of the striatum from METH self-administered rats, including those after 12 and 24 hours of abstinence.

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Transcriptional profiling of whisker follicles and of the striatum in methamphetamine self-administered rats

Project description:Transcriptional profiling of whisker follicles and of the striatum in methamphetamine self-administered rats

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Compulsive methamphetamine taking is associated with differential expression of HDAC2-regulated genes in the rat dorsal striatum

Project description:Methamphetamine (METH) use disorder (MUD) is a neuropsychiatric disorder with loss of self-control over drug intake despite of negative consequences. The behavioral consequences of long-term METH intake on compulsive and non-compulsive individuals remain unclear. Herein we trained rats to self-administered METH 1st for 22 days than 8 days of foot-shock and 15 days of abstinence. Which was followed by 12 days of reinstatement to METH self-administration then 3 days of foot-shock and 15 days of abstinence. After 1st foot-shock phase we found two phenotypes based on their METH intake i.e., shock resistance (SR, compulsive), and shock sensitive (SS, non-compulsive). Interestingly during reinstatement of METH self-administration then foot-shock, some (resurgent shock resistance, RSR) non-compulsive rats, displayed compulsive METH intake behavior. During both abstinence period rats were tested for drug seeking behavior and SR rats displayed higher METH seeking then RSR and SS. 24 hour after 2nd withdrawal day testing, we compared transcriptional and epigenetic changes in dorsal striatum.

2024-09-25 | GSE220896 | GEO

pCREB-dependent transcriptional activation is a mediator of molecular neuroadaptations induced by methamphetamine self-administration in the rat dorsal striatum

Project description:Neuroplastic changes in the dorsal striatum participate in the transition from casual drug use to habitual and compulsive drug taking. These alterations might also play a critical role in the development of methamphetamine (METH) addiction. Nevertheless, the molecular substrates that underlie habitual METH consumption have yet to be elucidated. Therefore, in the present study, we examined the influence of METH self-administration on the expression of genes and proteins of interest, as potential substrates of METH-induced neuronal plasticity in the dorsal striatum. Rats self-administered METH (0.1 mg/kg/injection, i.v.) during 15 h sessions for 8 d and were euthanized after 2 h, 24 h, or 1 month of abstinence. Compared to yoked saline control, METH self-administration induced increases in the mRNA expression of the transcription factors, c-fos and fosb, the neurotrophic factor, Bdnf, and of the synaptic protein, synaptophysin (Syp) at 2 h after cessation of drug exposure. METH self-administration also caused changes in FosB, BDNF and TrkB protein levels, with increases at 2 and 24 h but decreases observed after 1 month of drug abstinence. Importantly, METH exposure caused increases in the levels of H3K4me3 and pCREB after 2 and 24 h of abstinence. Chromatin immunoprecipitation followed by qPCR was used to clarify the role of these proteins in the regulation of gene expression. We found that METH self-administration caused enrichment of pCREB, but not of H3K4me3, on the promoters of c-fos, fosb, Bdnf and Syp at 2 h after drug cessation. These data indicate that METH-induced activation of their transcription is mediated, in part, by pCREB-dependent epigenetic phenomena. Thus, METH self-administration might trigger epigenetic changes that caused alterations in the expression of genes and proteins serving as substrates for addiction-related synaptic plasticity.

2012-06-01 | GSE38223 | GEO

RNA sequencing identified differentially expressed genes in the mesocorticolimbic and nigrostriatal systems of compulsive METH taking rats.

Project description:Methamphetamine (METH) is an extremely addictive drug which continues to cause significant harm to individuals and communities. Differences in patterns of abuse, amounts of METH taken, and in relapse rates exist among METH users who meet DSM-V criteria for METH use disorder (MUD). We are actively investigating the behavioral and molecular differences between compulsive and non-compulsive METH self-administering (SA) rats in the presence of a foot-shock which serves as an aversive stimulus. In the present study, we used male rats that were trained to self-administer METH (0.1 mg/kg/infusion, IV) on an FR-1 schedule for 20 days using a pattern of three 3-h sessions per day and followed by foot-shocks (0.18mA to 0.36mA) for the next 9 days. All the METH taking rats escalated their drug intake during the 20 days of training phase. During the foot-shock phase, a group of rats continued to self-administer METH in the presence of aversive stimuli and were called shock-resistant (SR, compulsive), whereas others who decreased their intake were termed shock-sensitive (SS, non-compulsive). Animals were euthanized 2 hours after the last SA session and the prefrontal cortex (PFC), nucleus accumbens (NAc), dorsal striatum (DStr) and midbrain (MBr) were isolated for RNA sequencing. RNA sequencing showed a large number of differentially expressed genes (DEGs) in all the brain regions. Our study supports the notion that studying multiple brain regions is critical for a comprehensive understanding of the interconnected neuroadaptive changes which are associated with substance use disorders (SUDs). The present study is of significant translational importance by further supporting the idea that targeting common DEGs identified across multiple brain regions might lead to the development of effective therapeutic approaches against MUD.

2025-09-30 | GSE301346 | GEO

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