Bacillus velezensis strain:YS-AT-DS1
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ABSTRACT:
PROVIDER: PRJNA869689 | ENA |
REPOSITORIES: ENA
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Project description:Root-knot nematodes (RKNs; Meloidogyne spp.), one of the most economically important plant-parasitic nematodes (PPNs), cause severe yield and quality losses in agriculture annually. The application of biological control agents is an environmentally safe and effective approach to control RKNs. Here, we report the genomic characteristics of a Bacillus velezensis strain YS-AT-DS1 (Bv-DS1) isolated from the tidal soil, revealing that it has a 4.73 Mb circular chromosome with an average GC-content of 46.43%, 3,977 genes, 86 tRNAs, and 27 rRNAs, and contains secondary metabolite clusters for producing antimicrobial compounds. In vitro assays indicated that Bv-DS1 has not only antagonistic activities against fungal pathogens, but also shows nematicidal activity, with a mortality rate of 71.62% mortality rates in second-stage juvenile (J2s) Meloidogyne incognita. We then focused on the biocontrol efficiency of Bv-DS1 against M. incognita in pot assays. Preinoculation with Bv-DS1 enhanced tomato growth, and significantly reduced the infection rate of J2s, and the number of galls and egg masses on tomato roots. The underlying mechanism in Bv-DS1-induced resistance to M. incognita was further investigated through split-root experiments, and analysing the expression of the genes related to jasmonic acid (JA), salicylic acid (SA), and the tonoplast intrinsic protein (TIP). The results indicated that Bv-DS1 could not activate host systemic-induced resistance (ISR) in the split-root system of tomatoes. Additionally, the expression of JA- (LOX D and MC) and SA- (PAL2 and PR) responsive genes did not change in Bv-DS1-pretreated plants at 3 and 14 days after nematode inoculation. The presented data showed that JA-and SA-dependent pathways were not required for the biocontrol action of the Bv-DS1 against RKN. The TIP genes, responsible for transport of water and small substrates in plants, have previously been shown to negatively regulate the parasitism of PPNs. Surprisingly, Bv-DS1 compromised the downregulation of TIP1.1 and TIP1.3 by M. incognita. Together, our data suggest that Bv-DS1 exhibits a dual effect on plant growth promotion and protection against RKN, possibly related to the regulation of water and solute transport via TIPs. Thus, the Bv-DS1 strain could be used as a biocontrol agent for RKN control in sustainable agriculture.
| S-EPMC9722970 | biostudies-literature
Project description:The present study aims to evaluate the response of the three Mediterranean local grapevines ‘Garnacha Blanca’, ‘Garnacha Tinta’, and ‘Macabeo’ to treatments with biocontrol products (BPs), a botanical extract (Akivi, Dittrichia viscosa extract) and a beneficial microorganism (Bacillus UdG, Bacillus velezensis). A combination of transcriptomics and metabolomics approaches were chosen in order to study grapevine gene expression and to identify gene marker candidates, as well as, to determine grapevine metabolites differentially concentrated in response to BPs treatments. Grapevine plants were cultivated in greenhouse controlled conditions and submitted to the treatments, and thereafter, leaves were sampled 24h after treatment to conduct gene expression study by RNA-sequencing for ‘Garnacha Blanca’ leaves extract and by RT-qPCR for the three cultivars. Differentially expressed genes (DEGs) were investigated for both treatments and highly influenced DEGs were selected to be tested in the three cultivars as treatment gene markers. In addition, extraction of leaf components was performed to quantify metabolites such as phytohormones, organic acids, and phenols. Considering all the upregulated and downregulated genes and enhanced metabolites concentrations, the treatments had an effect on jasmonic acid, ethylene, and phenylpropanoids defense pathways. In addition, several DEG markers were identified presenting a stable overexpression after the treatments in the three grapevine cultivars. These gene markers could be used to monitor the activity of the products in field treatments in future research. Further research will be necessary to confirm these first results under field conditions.
2022-12-18 | GSE211268 | GEO
Project description:Bacillus velezensis strain GH1-13 isolated from a rice paddy soil in Korea has been reported to promote plant growth and inhibit some pathogens. It contains a plasmid pBV71, thought to be of benefit to the strain, but there is no information on its effect. In order to elicit the plasmid effect on gene expression, mRNA and protein levels were analyzed at various stages of bacterial growth. Comparative gene expression profiles between the plasmid-containing and plasmid-free cells revealed that strain GH1-13 activated a transient stress response in the exponential phase. It showed early activation of expression of sigma W operon, liaIHGFSR operon, and transcription regulators for transition state, associated with carbon catabolite repression and secondary metabolite biosynthesis of acetoin, bacillaene, and macrolactin.
2021-05-17 | PXD008573 | Pride
Project description:To study microbial interaction effect on transcriptomic profile of B. velezensis 83, a comparative gene expression profiling analysis was performed.
2025-05-10 | GSE202842 | GEO
Project description:Bacillus velezensis strain:YCH92 | isolate:Bacillus velezensis Genome sequencing
| PRJNA1251308 | ENA
Project description:Lecanicillium fungicola, the causative agent of dry bubble disease on Agaricus bisporus results in significant crop losses for mushroom growers worldwide. Dry bubble disease is treated through strict hygiene control methods and the application of chemical fungicides but an increase in fungicide resistant L. fungicola strains has resulted in a need to develop alternative biocontrol treatment methods. The aim of the work presented here was to evaluate the response of L. fungicola to a Bacillus velezensis isolate to assess its potential as a novel biocontrol agent. The bacterial species in Serenade, a commercially available biocontrol treatment was also included in this analysis. Exposure of 48 hr L. fungicola cultures to 25% v/v 96h B. velezensis culture filtrate resulted in a 45% reduction in biomass (P < 0.0002) and a disruption in hyphal structure and morphology. Characterisation of the proteomic response of L. fungicola following exposure to B. velezensis culture filtrate revealed an increase in the abundance of a variety of proteins associated with stress response (Norsolorinic acid reductase (+8 fold), isocitrate lyase (+7 fold) and MMS19 nucleotide excision repair protein (+4 fold). There was also a decrease in the abundance of proteins associated with transcription (40S ribosomal protein S30 (-33 fold), 60S ribosomal protein L5 (-45 foldThe results presented here indicate that B. velezensis culture filtrate is capable of inhibiting the growth of L. fungicola and inducing a stress response, thus indicating its potential to control this important pathogen of mushrooms.
2022-06-01 | PXD028506 | Pride