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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [ChIP-seq]

ABSTRACT: Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [ChIP-seq]

PROVIDER: PRJNA870877 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21120534.fastq.gz	Fastqsanger.gz
	SRR21120535.fastq.gz	Fastqsanger.gz
	SRR21120536.fastq.gz	Fastqsanger.gz
	SRR21120537.fastq.gz	Fastqsanger.gz
	SRR21120538.fastq.gz	Fastqsanger.gz

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution.

Project description:Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution.

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [snRNA-seq]

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [snATAC-seq]

Project description:Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [snATAC-seq]

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [ChIP-seq]

Project description:During the maturation of myofibers, the expression and activity of the basic leucine zipper TF Maf are increased compared to developmental stages. To determine the gene program regulated by this TF, we performed ChIP-seq against Maf and H3K27ac in adult gastrocnemius and quadriceps skeletal muscles.

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution.

Project description:This SuperSeries is composed of the SubSeries listed below.

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [snRNA-seq]

Project description:To explore the mechanistic basis of the different phases of pre- and postnatal muscle development and the contraction-dependent control of muscle gene expression , we generated a multi-omics single nucleus RNAseq (snRNA-seq) and single nucleus ATAC-seq (snATAC-seq) atlas of normal muscle and paralyzed muscle from mice lacking Cacna1s.

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Opposing transcriptional circuits governing skeletal muscle development and maturation revealed at single nuclear resolution [snATAC-seq]

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Coordinated excitatory and inhibitory circuits governing unpredictable threat induced anxiety

Project description:Coordinated excitatory and inhibitory circuits governing unpredictable threat induced anxiety

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Coordinated excitatory and inhibitory circuits governing unpredictable threat induced anxiety

Project description:Anxiety is elicited by excessive apprehension about unpredictable threats. However, the neural circuit governing unpredictable threat induced anxiety remains unclear. Here, we found ventral bed nucleus of the stria terminalis (vBNST) GABAergic neurons displayed selective activation to unpredictable threats by means ofthrough coordinated excitatory input from insular cortex (IC) glutamergic neurons and inhibitory input from lateral nucleus of the amygdala (CeL) somatostatin (SOM) neurons. Using activity-dependent neuronal tagging technology, we found that unpredictable threat responsive cells in vBNST drive freezing and anxiety via projections to ventral lateral periaqueductal grey (vlPAG) and median nucleus of the amygdala (CeM) respectively. Finally, we identified KCNQ3 plays an essential role in hyperactivity of vBNST GABAergic neurons and induced anxiety. These data identified a forward inhibitory circuit that determine the selective activation of vBNST in unpredictable threat and anxiety, and suggest that Kcnq3 KCNQ3 channel acts as a promising target in treatment of anxiety disorder following unpredictable stress.

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Single-cell activation screen identifies hepatic maturation regulators with zonal resolution

Project description:The maturation of lineage-committed embryonic hepatocytes requires both the timed activation of metabolic gene regulatory networks (GRNs) and silencing of embryonic programs to achieve adult hepatic functions. However, in vitro derivation of mature hepatocytes remains imperfect, and key transcriptional regulators governing GRN rewiring during late development are still insufficiently defined. To address this, we generated a developmental reference atlas and employed a dCas9 activation screen with single-cell transcriptomics on primary mouse embryonic hepatocytes, enabling effect ranking among late-onset transcription regulators. We identify Nr1i3 as a potent inducer of pericentrally expressed metabolic genes and Nfix as a critical suppressor of embryonic and periportal signatures. Supplementing liver zonation patterning signals with these regulators further enhanced the expression of pericentrally zonated metabolic genes, emphasizing the importance of a microenvironment-targeted approach. Our screening and analysis therefore highlight regulatory mechanisms underlying organ maturation and offer general strategies for improving the functionality of in vitro-derived cells.

2025-09-05 | GSE242934 | GEO

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