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Homo sapiens

ABSTRACT: Disulfiram/Copper induces immunogenic cell death via NPL4 in hepatocellular carcinoma

PROVIDER: PRJNA871352 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21146500_1.fastq.gz	Fastqsanger.gz
	SRR21146500_2.fastq.gz	Fastqsanger.gz
	SRR21146501_1.fastq.gz	Fastqsanger.gz
	SRR21146501_2.fastq.gz	Fastqsanger.gz
	SRR21146502_1.fastq.gz	Fastqsanger.gz

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Disulfiram/Copper induces immunogenic cell death via NPL4 in hepatocellular carcinoma

Project description:Some chemotherapeutic agents have been found to enhance the antitumor immunity by inducing immunogenic cell death (ICD). The combination of disulfiram (DSF) and copper (Cu) has demonstrated anti-tumor effects in a range of malignancies including hepatocellular carcinoma (HCC). However, the potential of DSF/Cu as an ICD inducer and whether it could enhance the efficacy of immune checkpoint blockade in HCC remains unknown. Here, we showed that DSF/Cu-treated HCC cells exhibited characteristics of ICD in vitro, such as calreticulin (CRT) exposure, ATP secretion, high mobility group box 1 (HMGB1) release. DSF/Cu treated HCC cells elicited significant immune memory in a vaccination assay. DSF/Cu treatment promoted dendritic cell activation and maturation. The combination of DSF/Cu and CD47 blockade further facilitated DC maturation and subsequently enhanced CD8+ T cell cytotoxicity. Mechanically, DSF/Cu promoted the nuclear accumulation and aggregation of nuclear protein localization protein 4 (NPL4) to inhibit the ubiquitin-proteasome system, thus induced endoplasmic reticulum (ER) stress. Inhibition of NPL4 induced ICD-associated damage-associated molecular patterns. Collectively, our findings demonstrated that DSF/Cu induced ICD-mediated immune activation in HCC and enhanced the efficacy of CD47 blockade.

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Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [RNA-Seq]

Project description:Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [RNA-Seq]

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Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [ChIP-Seq]

Project description:Isoginkgetin induces autophagic cell death in hepatocellular carcinoma [ChIP-Seq]

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Effect of S4 treatment on glioma cells

Project description:S4, a sulfonamide drug, has been confirmed to induce apoptosis and autophagy in cancer cells. Immunogenic cell death is a special cell death type which is closely related to apoptosis and autophagy. We performed RNA-seq to determine the impact of S4 on global gene expression profile in LN229 cells. Our results show that S4 induces immunogenic cell death via the response to endoplasmic reticulum stress.

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Project description:Disulfiram/copper exhibits anti-tumor activity against hepatocellular carcinoma via FTO/m6A/IGF2BP2/FDX1 signaling mediating cuproptosis

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Gene expression changes in SHI-1 cells following exposure to disulfiram/copper, niclosamde or combined drugs

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