Project description:India has a rich diversity of flora with a vast collection of medicinal plants. About 7000 flowering plants of India are reported with medicinal properties. However, research on medicinal plants is lacking in their molecular aspect. In order to generate and investigate the molecular resources of an ethno-medicinally important Allium species i.e., Allium hookeri, commonly known as hooker chives, we perform transcriptomic profiling of the herb. The involvement of miRNAs in metabolites regulation in A. hookeri is also anticipated. In the present study, we have de novo assembled and characterized the transcriptome of hooker chives roots using Illumina paired-end sequencing technology and searched for genes from the assembled transcripts. Furthermore, we have identified miRNAs from the plant and tried to co-relate with the transcripts. The discovery of these miRNA:target pairs will provide a basis for further understanding of their function in its growth and development.