ABSTRACT: Microbial community from a sequencing batch reactor enriching for polyphosphate accumulating organisms of the genus "Ca. Accumulibacter" Raw sequence reads
Project description:Polyphosphate accumulating organisms are responsible for enhanced biological phosphate removal from wastewater, where they grow embedded in a matrix of extracellular polymeric substances. Little is known about the composition and dynamics of those proteins and their production by the different microorganisms. Tomás-Martínez et al., (2022) studied the turnover of proteins and polysaccharides in extracellular polymeric fractions of an enrichment culture of polyphosphate accumulating organisms using an anaerobic-aerobic sequencing batch reactor simulating EBPR conditions. Finally, the carbon source was switched to 13C-labelled acetate to study the protein turnover. Samples were collected at the end of each aerobic phase.
2022-12-25 | PXD036522 | Pride
Project description:Microbial community from sequencing batch reactors enriching for "Ca. Accumulibacter"
Project description:Transcriptional profiling of the Donna II mixed community containing Dehalococcoides mccartyi strain 195 comparing a batch starved control to the mixed community being fed 1,2,3,4-tetrachlorobenzene as an electron acceptor. The goal was to determine which transcripts are regulated in response to a shift in a different electron acceptor rather than the consistent tetrachloroethene (PCE) that the parent reactor was maintained on.
Project description:In this study, a complex microbial community from a semi-continues reactor, which only substrate is wheat straw, was incubated in a batch experiment with 13C-cellulose. protein stable isotope probing (protein-SIP) was used to identify the organisms, at high taxonomic resolution, involved in the degradation of cellulose by tracking the incorporation of 13C in the newly synthetized proteins. Thereby providing information regarding identity and function simultaneously and enable the optimization of biotechnologies for biofuels production.
Project description:Extracellular proteins play key roles in microbial biofilm and aggregate formation, yet remain poorly understood. Using two advanced metaproteomic techniques, this study identified and classified 387 extracellular proteins potentially involved in aggregate formation, including filamentous and cell surface proteins from various organisms. The findings suggest that diverse proteins from multiple organisms support the granular biofilm formation in the investigated Ca. Accumulibacter enrichment, providing a valuable resource for studying the scope of extracellular proteins.
Project description:A metabolomics and whole cell lysate shotgun proteomics study was performed to investigate the glycolytic modes in Ca. Accumulibacter phosphatis.
2024-10-28 | PXD048326 | Pride
Project description:Microbial community of anaerobic sequence batch reactor