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AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis

ABSTRACT: AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis

PROVIDER: PRJNA876101 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21397300.fastq.gz	Fastqsanger.gz
	SRR21397301.fastq.gz	Fastqsanger.gz
	SRR21397302.fastq.gz	Fastqsanger.gz
	SRR21397303.fastq.gz	Fastqsanger.gz
	SRR21397304.fastq.gz	Fastqsanger.gz

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Gas6/Axl signaling activates Akt in liver cancer cells

Project description:We investigated which signaling pathways downstream of Gas6/Axl promote the invasive phenotype of liver cancer cells. Thus, we performed phospho-proteomic analysis of MR hepatocytes and those expressing Axl (MR-Axl) in the presence and absence of Gas6. Protein-clustering based on the pattern of phosphorylation across the conditions and samples revealed four clusters: phospho-sites that are upregulated dependent on Axl expression (cluster 1) or Gas6 expression (cluster 2) and phospho-sites that are downregulated dependent on Axl expression (cluster 3) or Gas6 expression (cluster 4). Functional enrichment analysis showed enrichment of protein sets associated with cellular polarity and motility depending on changes in Axl expression and Gas6 activation. Furthermore, we analyzed phospho-proteomic data to estimate the activity of kinases based on kinase-substrate interactions using PhosR. Kinase perturbation analysis indicated mammalian target of rapamycin (mTOR) as the one with the highest upregulated activity in Gas6-stimulated MR-Axl cells compared to Gas6-stimulated MR cells. Accordingly, we analyzed substrates of mTOR and found that phosphorylation of Akt1 (Ser473) was upregulated in cluster 1 and highly increased in Gas6-stimulated MR-Axl, suggesting that Akt is regulated by Axl.

2024-05-23 | PXD047577 | Pride

AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis [scRNA-Seq]

Project description:AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis [scRNA-Seq]

| PRJNA876108 | ENA

AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis [bulkRNA-Seq]

Project description:AA2P-mediated DNA demethylation synergizes with stem cell agonists to promote expansion of stem cells via activation of GAS6/AXL signaling axis [bulkRNA-Seq]

| PRJNA876104 | ENA

Gas6-Axl signaling drives oral regenerative wound repair

Project description:Rapid and scarless wound repair is a hallmark of the oral mucosa, yet the cellular and molecular mechanisms that enable this regeneration remain unclear. By comparing populations of oral mucosal fibroblasts (OMFs) and facial skin fibroblasts (FSFs), we have identified the mechanisms that facilitate regeneration over fibrosis. We found that OMFs utilize Growth arrest specific-6 (GAS6)-AXL signaling to suppress fibrosis-related mechanosignaling via Focal adhesion kinase (FAK). Inhibition and knockdown of AXL in the oral mucosa resulted in fibrotic wounds and increased activation of FAK. At the same time, stimulation of AXL in the facial skin yielded wounds that heal regeneratively. Rare human oral scars that resulted from repetitive injury showed decreased expression of GAS6/AXL and increased FAK. Activating AXL in repetitively injured mouse oral tissue resulted in better wound healing outcomes and reduced scarring. Together, our work demonstrates that AXL signaling is necessary for regenerative wound healing in the oral mucosa and sufficient to limit facial skin fibrosis.

2025-04-30 | GSE287384 | GEO

Effect of Gas6/Axl on HCC cell line gene expression

Project description:We adressed the impact of the receptor tyrosine kinase Axl and ist ligand Gas6 on gene expression in cellular HCC models, including cells harbouring Axl KO and exogenous reexpression.

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Quantitative tyrosine phosphoproteome profiling of GAS6-AXL signaling network

Project description:Overexpression and amplification of AXL receptor tyrosine kinase (RTK) has been found in several hematologic and solid malignancies. Activation of AXL by its ligand GAS6 can enhance tumor-promoting processes such as proliferation, migration, invasion, and survival. Despite the important role of AXL in cancer development, a deep and quantitative mapping of its signaling transduction has not been performed. Here, we used a mass spectrometry-based quantitative proteomic approach to characterize the temporal dynamics of the phosphotyrosine proteome of MDA-MB-231 cells stimulated with GAS6

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Gas6-Axl signaling drives oral regenerative wound repair [bulk RNA]

2025-04-30 | GSE287839 | GEO

Gas6-Axl signaling drives oral regenerative wound repair [RNA-Seq]

2025-04-30 | GSE287947 | GEO

Gas6-Axl signaling drives oral regenerative wound repair [scRNA-seq]

2025-04-30 | GSE287528 | GEO

Gas6-Axl signaling drives oral regenerative wound repair

Project description:Gas6-Axl signaling drives oral regenerative wound repair

| PRJNA1212540 | ENA