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Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

ABSTRACT: Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

PROVIDER: PRJNA877228 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21440800_1.fastq.gz	Fastqsanger.gz
	SRR21440800_2.fastq.gz	Fastqsanger.gz
	SRR21440801_1.fastq.gz	Fastqsanger.gz
	SRR21440801_2.fastq.gz	Fastqsanger.gz
	SRR21440802_1.fastq.gz	Fastqsanger.gz

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Effect of NCOA4-depletion on the transcriptome profile of J774 mouse macrophage cells

Project description:To determine the physiological roles of NCOA4 and ferritinophagy in macrophages, NCOA4 was depleted via liposome-mediated siRNA delivery into cells. Cells were treated with siRNA for NCOA4 depletion and then the transcriptome profiles acquired from RNA-seq were compared with those of control (scramble siRNA-treated) cells.

2022-09-30 | GSE212772 | GEO

Effect of iron depletion on gene expression of NCOA4-deficient and control HT22 hippocampal neuron cells

Project description:To determine the physiological roles of NCOA4 and NCOA4-mediated ferritinophagy in hippocampal neurons, NCOA4 was depleted using siRNA and desferrioxamine (DFO; 100 µM, 24 h) was added to restrict iron availability. Cells were treated for NCOA4 depletion, iron restriction, or both, and then the transcriptome profiles acquired from RNA-seq were compared with those of control (scramble siRNA-treated) cells.

2022-12-30 | GSE211931 | GEO

NCOA4

Project description:NCOA4

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Transcriptome profiling of human erythroblast cells knocking down NCOA4

Project description:Purpose: Knocking down NCOA4 disrupted Thyroid hormone receptor beta agonist,GC-1, mediated terminal human erythroblast differentiation. Therefore, we conducted RNA-seq to profile the transcriptome changes in NCOA4 knocked down human erythroblasts.. Methods: Human CD34+ cells were transduced by lentiviruses encoding shRNAs targeting either LacZ (control) or NCOA4 atday 1 of culture. At day 14, cells were switched to terminal differentiation medium. Results: Using an optimized data analysis workflow, we have identified an ensemble of genes whose expression are discrupted by NCOA4 knockdown. Conclusions: There is a significant correlation between the degree of GC-1-mediated gene activation and the degree of NCOA4 knockdown-mediated gene repression.

2017-09-06 | GSE102582 | GEO

Effect of depletion of Olfml3 on macrophage function

Project description:Effect of depletion of Olfml3 on macrophage function

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The effect of talc on J774 cells

Project description:The effect of talc on J774 cells

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Effect of iron depletion on gene expression of NCOA4-deficient and control HT22 hippocampal neuron cells

Project description:Effect of iron depletion on gene expression of NCOA4-deficient and control HT22 hippocampal neuron cells

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Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis

Project description:Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particularly for host-pathogen interaction studies, is debatable. Here we use high-throughput RNA-sequencing to analyse transcriptome dynamics of two macrophage models in response to M. tuberculosis infection. Specifically, we study the early response of bone marrow-derived mouse macrophages and cell line J774 to infection with live and -irradiated (killed) M. tuberculosis. We show that infection with live bacilli specifically alters the expression of host genes such as RSAD2, IFIT1/2/3 and RIG-I, whose potential roles in resistance to M. tuberculosis infection have not yet been investigated. In addition, the response of primary macrophages is faster and more intense than that of J774 cells in terms of number of differentially expressed genes and magnitude of induction/repression. Our results point to potentially novel processes leading to immune containment early during M. tuberculosis infection, and support the idea that important differences exist between primary macrophages and cell lines, which should be taken into account when choosing a macrophage model to study host-pathogen interactions.

2016-10-18 | GSE88801 | GEO

The effect of macrophage depletion on mammary basal cells

Project description:The effect of macrophage depletion on mammary basal cells

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The effect of macrophage depletion on mammary basal cells

Project description:To investigate the effect of macrophages on mammary basal cells, we established a macrophage depletion mouse model by clodronate liposome (CL) intraperitoneal injection, and the mammary basal cells were collected by FACS sorting. We then performed RNA-seq analysis on clodronate liposome (CL) treated mammary basal cells compared to control.

2023-04-20 | GSE210268 | GEO

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