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Effect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells

ABSTRACT: Effect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells

PROVIDER: PRJNA877646 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21465858.fastq.gz	Fastqsanger.gz
	SRR21465859.fastq.gz	Fastqsanger.gz
	SRR21465860.fastq.gz	Fastqsanger.gz
	SRR21465861.fastq.gz	Fastqsanger.gz
	SRR21465862.fastq.gz	Fastqsanger.gz

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Effect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells

Project description:To investigate the effect of changes in oxygen tension and treatment with cyclosporine A on the chemotactic migration of B cells, we grew RAMOS B cells at 19% and 1% oxygen levels, and at 19% and 1% oxygen levels after treatment with cyclosporine A. We then performed RNA-seq on these RAMOS B cells and subequently gene expression profiling analysis on this RNA-seq data.

2023-02-13 | GSE212853 | GEO

Effect of hypoxia and treatment with cyclosporine A on chemotaxis of RAMOS B cells

Project description:Background: Hypoxia is a potent molecular signal for cellular metabolism, mitochondrial function, and migration. Conditions of low oxygen tension trigger regulatory cascades mediated via the highly conserved HIF-1 α post-translational modification system. In the adaptive immune response, B cells (Bc) are activated and differentiate under hypoxic conditions within lymph node germinal centers, and subsequently migrate to other compartments. During migration, they traverse through changing oxygen levels, ranging from 1-5% in the lymph node to 5-13% in the peripheral blood. Interestingly, the calcineurin inhibitor cyclosporine A is known to stimulate prolyl hydroxylase activity, resulting in HIF-1 α destabilization and may alter Bc responses directly. Over 60% of patients taking calcineurin immunosuppressant medications have hypo-gammaglobulinemia and poor vaccine responses, putting them at high risk of infection with significantly increased morbidity and mortality. Results: We demonstrate that oxygen tension is a previously unrecognized Bc regulatory switch, altering CXCR4 and CXCR5 chemokine receptor signaling in activated Bc through HIF-1 α expression, and controlling critical aspects of Bc migration. Our data demonstrate that calcineurin inhibition hinders this oxygen regulatory switch in primary human Bc. Conclusion: This previously unrecognized effect of calcineurin inhibition directly on human Bc has significant and direct clinical implications.

2023-07-20 | PXD037004 | Pride

Effect of expression of ZEBRA on gene expression during Ramos cells

Project description:EBV immediate early protein ZEBRA was corroborated to interact with Pax5 which controls the fate of B cells. Ramos cells were infected with ZEBRA-expression lentivirus and positively infected cells were sorted, which were named Ramos-Lv-ZEBRA.

2024-04-24 | GSE264391 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Ramos and Rituximab-resistant Ramos cell Transcriptomes

Project description:The goal of this study is to screen the differential genes between Ramos and Rituximab-resistant Ramos cells, then analyze the significant pathways.

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Effect of expression of ZEBRA on gene expression in Ramos cells [CUT-Tag]

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Effect of expression of ZEBRA on gene expression in Ramos cells [ChIP-Seq]

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Gene expression in Ramos human B cell line induced by treatment with anti-FCRL1 antibody treatment

Project description:B cell receptor CD40 and FCRL1 are highly expressed in Ramos B cell line. Therefore, these receptors are considered to cooperate in B cell activation. In this study, we compared gene expression profiles induced in Ramos cell line by combined treatments of anti-IgM antibody, anti-CD40 antibody, and anti-FCRL1 antibodies.

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Cyclosporine on fibroblast

Project description:To elucidate the gene profile of anti-fibroproliferative effects of cyclosporine, we added TGF-b and/or CsA to MRC5 (fetal human lung fibroblast) cell line. After 24 hours serum starvation, fibroblasts were treated with 3 ng/ml TGF-β1 and were treated with or without 2μg/ml Cyclosporine and effects were examined at 48 hours after treatment. Expression of three genes (IGFBP3, ID1 and PPARG) from this signature was quantified in the same RNA samples by real-time PCR. TGF-β and cyclosporine induced gene expression data was measured in MRC5 (fetal human fibroblast cell line) at 48h after treatments. The dose of TGF-β was 3 ng/ml and cyclosporine was 2 μg/ml. The comparison was done between four groups (control, TGF-β, cyclosporine and TGF-β plus cyclosporine).Three independent experiments were performed at each treatments using different samples for each experiment.

2010-11-09 | E-GEOD-25200 | biostudies-arrayexpress

Cyclosporine on fibroblast

2010-11-09 | GSE25200 | GEO

Gene expression profiles of Ramos tumor cells with TNFRSF17 knockdown.

Project description:TNFRSF17 in Ramos tumor cells has been found to affect their interaction with CD19 CAR T cells. We used RNA sequencing to compare the transcriptomic profiles between TNFRSF17 knockdown Ramos cells and unperturbed control cells.

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