Project description:The aim of this study was to compare the tomato global transcriptional profiles in response to host attack by ToMV and Fol in order to identify genomic differences and similarities in incompatible interactions between a foliar and a vascular pathogen. In order to identify a set of genes of interest in tomato plants infected with F. oxysporum f. sp. lycopersici (Fol) and Tomato Mosaic Virus (ToMV) a transcriptional analysis was performed. Tomato genes differentially expressed upon inoculation with Fol and ToMV were identified at 2 days post-inoculation, using an un-inoculated sample as reference.
Project description:The aim of this study was to compare the tomato global transcriptional profiles in response to host attack by ToMV and Fol in order to identify genomic differences and similarities in incompatible interactions between a foliar and a vascular pathogen.
Project description:21 DPI Marchantia polymorpha Tak-1 thalli were infected with Fol WT and erc3 knock out alongside mock(water) control and axenically grown Fol WT culture. At 3 days post infection, thalli were harvested and RNA was extracted. After polyA enrichment, and library preparation, sequencing was carried on NovaSeq6000 platform using SP flowcell with 2 x 100 bp read length.
