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Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations

ABSTRACT: Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations

PROVIDER: PRJNA880870 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21593900.fastq.gz	Fastqsanger.gz
	SRR21593901.fastq.gz	Fastqsanger.gz
	SRR21593902.fastq.gz	Fastqsanger.gz
	SRR21593903.fastq.gz	Fastqsanger.gz
	SRR21593904.fastq.gz	Fastqsanger.gz

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Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations

Project description:The mammary gland is a dynamic tissue that mainly develops postnatally under the influence of fluctuating hormone levels. This dataset contains bulk RNAseq data of different mouse mammary gland cell populations (basal, luminal and stromal) that were isolated either during puberty or at different stages of the adult virgin estrous cycle. These data are of interest to researchers in the mammary gland and breast cancer biology field as they allow studies on spatiotemporal, lineage specific changes in gene expression.

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Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations.

Project description:Conflicting data exist as to how mammary epithelial cell proliferation changes during the reproductive cycle. To study the effect of endogenous hormone fluctuations on gene expression in the mouse mammary gland, we performed bulk RNAseq analyses of epithelial and stromal cell populations that were isolated either during puberty or at different stages of the adult virgin estrous cycle. Our data confirm prior findings that proliferative changes do not occur in every mouse in every cycle. We also show that during the estrous cycle the main gene expression changes occur in adipocytes and fibroblasts. Finally, we present a comprehensive overview of the Wnt gene expression landscape in different mammary gland cell types in pubertal and adult mice. This work contributes to understanding the effects of physiological hormone fluctuations and locally produced signaling molecules on gene expression changes in the mammary gland during the reproductive cycle and should be a useful resource for future studies investigating gene expression patterns in different cell types across different developmental timepoints.

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