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Differential transcriptomic expression of an imprved astaxanthin production

ABSTRACT: Differential transcriptomic expression of an imprved astaxanthin production

PROVIDER: PRJNA880907 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21595131_1.fastq.gz	Fastqsanger.gz
	SRR21595131_2.fastq.gz	Fastqsanger.gz
	SRR21595132_1.fastq.gz	Fastqsanger.gz
	SRR21595132_2.fastq.gz	Fastqsanger.gz
	SRR21595133_1.fastq.gz	Fastqsanger.gz

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Differential transcriptomic expression of an imprved astaxanthin production

Project description:The differential expression of the high-yield astaxanthin Phaffia rhodozyma BPAX-A1 with the wild-type Phaffia rhodozyma CBS6938 was obtained and analysed to understand metabolic changes leading to an improved astaxanthin production.

2023-09-14 | GSE213476 | GEO

Salicylic acid treatment and overexpression of a novel polyamine transporter gene for astaxanthin production in Phaffia rhodozyma

Project description:Phaffia rhodozyma is an ideal microbial astaxanthin resource. However, the low productivity of astaxanthin in this yeast obstructs the process of industrial production. Although P. rhodozyma is isolated from plant material and the phytohormone has proved to be an effective stimulator for microbial production, the effects and mechanisms of phytohormones on astaxanthin synthesis in P. rhodozyma have been rarely reported. In this study, a concentration of down to 0.5 mg/L exogenous salicylic acid (SA) could promote biomass, astaxanthin content and yield by 20.8%, 95.8% and 135.3% in P. rhodozyma, respectively. Further transcriptomic analysis showed that SA could discriminate well the transcriptomic profile of P. rhodozyma cells. The differently expressed genes (DEGs) in P. rhodozyma cells between the SA-treatment and the SA-free groups were identified, and involved in astaxanthin and its competitive metabolite synthesis, material supply, biomolecules metabolism and transportation, anti-stress and signal transductions at a global-cell level. A regulation mechanism of astaxanthin synthesis induced by SA, including perception and transduction of the SA signal, expression regulations of the downstream genes by transcription factors, and cellular stress response to the SA, is put forward in this study. The polyamine transporter gene (PT), as an up-regulated DEG, was overexpressed in the P. rhodozyma to obtain the transformant Prh-PT-006. It was found that biomass, astaxanthin content and yield of the Prh-PT-006 under 0.5 mg/L SA treatment could reach 6.6 g/L, 0.35 mg/g DCW and 2.3 mg/L, 24.5%, 143.1% and 199.0% higher than the wild strain at the SA-free condition, respectively. The result will provide the engineering targets for constructing strains with high-yield of astaxanthin, and help to accelerate the industrialization process of microbial astaxanthin.

2023-10-16 | GSE242572 | GEO

Comparative metabolic and transcriptomic analysis of macrozoids and palmelloid cells of Haematococcus pluvialis related to astaxanthin accumulation

Project description:In this work, we propose a metabolomic and transcriptomic comparative study between a vegetative state and intermediate reddish palmelloid state growing in continuous culture conditions, giving rise to a profound understanding of the first steps of the adaptation of Haematococcus pluvialis to nitrogen limitation and the correlation with astaxanthin biosynthesis. In addition, other related metabolic pathways as fatty acid biosynthesis, starch accumulation, citric acid cycle, and amino acid biosynthesis have been analyzed. Understanding the molecular basis of astaxanthin accumulation will be useful for the optimization of astaxanthin production.

2021-04-07 | GSE161337 | GEO

Hepatic transcriptome profiles of mice with diet-induced nonalcoholic steatohepatitis treated with astaxanthin and vitamin E

Project description:Astaxanthin alleviates hepatic lipid accumulation and peroxidation, inflammation, and fibrosis in mice with high-cholesterol, high-cholate, and high-fat (CL) diet-induced nonalcoholic steatohepatitis (NASH). It has been proposed as a potential new treatment to inhibit the progression of NASH in humans. Therefore, we compared hepatic gene expression profiles after treatment with astaxanthin or the antioxidant vitamin E in mice with CL diet-induced NASH. Comprehensive gene expression analyses of the livers of mice fed a standard, CL, or CL diet containing astaxanthin or vitamin E for 12 weeks were performed using a DNA microarray. Both astaxanthin and vitamin E effectively improved gene expression associated with eukaryotic initiation factor-2 (EIF2) signaling, which is suppressed in NASH by endoplasmic reticulum (ER) stress in the liver. Astaxanthin but not vitamin E was predicted to suppress the actions of ligand-dependent nuclear receptors peroxisome proliferator-activated receptors (PPAR) α and PPARδ and to affect related molecules. Establishing a new therapy using astaxanthin will require elucidation of astaxanthin’s molecular action on the functions of PPARα and related molecules in the livers of mice with diet-induced NASH.

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Plasma-based proteomics analysis of molecular pathways in canine diabetes mellitus after astaxanthin supplementation

Project description:The hyperglycemic state in diabetes mellitus (DM) induces oxidative stress and inflammation, thus contributing to diabetic tissue damage and associated complications. Astaxanthin, a potent antioxidant carotenoid, has been investigated for its potential in preventing and managing diabetes across various species, but its effect on client-owned dogs is not well-studied. This study explored the impact of astaxanthin supplementation on canine DM using a proteomic approach. A total of 18 client-owned dogs were enrolled: 6 dogs with DM and 12 clinically healthy dogs. The diabetic dogs received their standard treatment regimen alongside daily oral supplementation of 12 mg of astaxanthin (1.5–2.4 mg/kg) for 90 days. Plasma samples were collected at the beginning and end of the study period for proteomic analysis. After astaxanthin supplementation, there were significant alterations in protein expression associated with the complement system, coagulation cascade, JAK-STAT signaling, and protein kinase C signaling, which all contribute to inflammation and oxidative stress. Astaxanthin demonstrated a protective effect against diabetes-associated complications, including insulin resistance, vascular dysfunction, nephropathy, and cardiac complications. These findings highlight the potential of astaxanthin as a complementary therapeutic agent for controlling DM in canines.

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Astaxanthin Supplementation Counters Exercise-Induced Decreases in Immune- Related Plasma Proteins

Project description:Astaxanthin is a dark red keto-carotenoid found in aquatic animals such as salmon and shrimp, and algae (Haematococcus pluvialis). Astaxanthin has a unique molecular structure that may facilitate anti-oxidative, immunomodulatory, and anti-inflammatory effects during physiological stress. The primary objective of this study was to examine the efficacy of 4-weeks ingestion of astaxanthin in moderating exercise-induced inflammation and immune dysfunction using a multi-omics approach.

2023-05-10 | PXD040503 | Pride

Transcriptomic changes induced by salinity stress in the green microalga Chromochloris zofingiensis

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Transcriptome analysis in Haematococcus pluvialis: astaxanthin induction by salicylic acid (SA) and jasmonic acid (JA)

Project description:Haematococcus pluvialis transcriptome analysis The genetic transcriptome details of astaxanthin biosynthesis were analysed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). SRP062153 PRJNA292140

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