Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice I

ABSTRACT: Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice I

PROVIDER: PRJNA882090 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21629067_1.fastq.gz	Fastqsanger.gz
	SRR21629067_2.fastq.gz	Fastqsanger.gz
	SRR21629068_1.fastq.gz	Fastqsanger.gz
	SRR21629068_2.fastq.gz	Fastqsanger.gz
	SRR21629069_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice II

Project description:In mammalian embryos, Dux transcription factors (TF) drive a cleavage stage-specific transcriptional burst associated with zygotic genome activation (ZGA) during which hundreds of genes and endogenous retroviral (ERV) elements are transiently expressed. In mice, ZGA begins in late 1-cell (1C) embryos and is shut down within hours at the late 2-cell (2C) to 4-cell (4C) stage. While this transition is accompanied by the loss of totipotency, it is not clear whether shutdown of Dux-induced ZGA is required for this process and how ZGA is terminated. Here, we reveal an essential negative feedback axis by which the Dux family member Duxbl terminates Dux-induced ZGA from the mid 2C stage onward by direct suppression of genomic Dux target loci. Consequently, Duxbl inactivation results in sustained expression of Dux-induced ZGA leading to 4C arrest. Our study reveals the Dux/Duxbl axis that determines the timing of totipotency exit enabling the very first divergence of cell fates.

2023-12-07 | GSE213701 | GEO

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice I

2023-12-07 | GSE213700 | GEO

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice III

2023-12-07 | GSE213702 | GEO

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice

Project description:Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice

| PRJNA882084 | ENA

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice II

Project description:Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice II

| PRJNA882092 | ENA

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice III

Project description:Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice III

| PRJNA882091 | ENA

The homeobox transcription factor DUXBL controls exit from totipotency

Project description:In mice, exit from the totipotent 2-cell (2C) embryo stage requires silencing of the 2C-associated transcriptional program. However, the molecular mechanisms involved in this process remain poorly understood. Here, we demonstrate that the 2C-specific transcription factor DUX mediates an essential negative feedback loop by inducing the expression of DUXBL to promote this silencing.We show that DUXBL gains accessibility to DUX-bound regions specifically upon DUX expression.Furthermore, we determine that DUXBL interacts with TRIM24 and TRIM33, members of the tripartite motif superfamily involved in gene silencing and co-localizes with them in nuclear foci upon DUX expression. Importantly, DUXBL overexpression impairs 2C-associated transcription, whereas Duxbl inactivation in ESC increases DUX-dependent induction of the 2C-transcriptional program. Consequently, DUXBL deficiency in embryos results in sustained expression of 2C-associated transcripts, leading to early developmental arrest.Our study identifies DUXBL as an essential regulator of totipotency exit enabling the first divergence of cell fates.

2023-12-07 | GSE234053 | GEO

Duxbl-mediated suppression of ZGA drives totipotency to pluripotency transition in mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-12-07 | GSE213703 | GEO

The homeobox transcription factor DUXBL controls exit from totipotency

Project description:The homeobox transcription factor DUXBL controls exit from totipotency

| PRJNA979244 | ENA

Brg1 defect causes aberrent nucleosome landscape and affects totipotency & pluripotency transcriptional factors binding during embryogenesis

Project description:Mammalian embryogenesis involves intricate epigenetic reprogramming and chromatin remodeling. In this study, we investigated the role of Brg1, a catalytic ATPase subunit of the SWI/SNF complex, in early embryonic development and mouse embryonic stem cell (mESC) pluripotency. By generating Smarca4 flox/flox Gdf9-Cre female mice to knockout Brg1 in MII oocytes and using CRISPR-Cas9 to knockout Brg1 in mESCs, we found that Brg1 deficiency led to multiple defects. In mouse embryos, maternal knockout of Brg1 caused reduced blastocyst formation, delayed development, elevated nucleosome occupancy at gene promoters, and dysregulation of zygotic genome activation (ZGA) genes, potentially due to disrupted binding of totipotency-related transcription factors (TFs). In mESCs, Brg1 knockout resulted in abnormal clone morphology, decreased pluripotency marker expression, and reduced cell proliferation. Brg1 affected pluripotency mainly by influencing the binding of pluripotency-related TFs such as Oct4 through enhancer accessibility. We identified those TFs as totipotency/pluripotency Brg1-dependent TFs. Overall, this study demonstrates the crucial role of Brg1 in ZGA and mESC identity maintenance in affecting specific TF binding on proximal and distal regulatory elements.

2025-05-09 | GSE295748 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data