Actitis macularius
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ABSTRACT:
PROVIDER: PRJNA882932 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Eublepharis macularius Genome sequencing and assembly
| PRJNA339626 | ENA
Project description:Eublepharis macularius transcriptome to identify immunoglobulins
| PRJNA308941 | ENA
Project description:RNA-Seq from liver of Eublepharis macularius
| PRJDB3879 | ENA
Project description:A revised leopard gecko (Eublepharis macularius) reference genome
| PRJNA884264 | ENA
Project description:Eublepharis macularius strain:EM1126 Genome sequencing
| PRJNA1223145 | ENA
Project description:BackgroundGeckos are among the most species-rich reptile groups and the sister clade to all other lizards and snakes. Geckos possess a suite of distinctive characteristics, including adhesive digits, nocturnal activity, hard, calcareous eggshells, and a lack of eyelids. However, one gecko clade, the Eublepharidae, appears to be the exception to most of these 'rules' and lacks adhesive toe pads, has eyelids, and lays eggs with soft, leathery eggshells. These differences make eublepharids an important component of any investigation into the underlying genomic innovations contributing to the distinctive phenotypes in 'typical' geckos.FindingsWe report high-depth genome sequencing, assembly, and annotation for a male leopard gecko, Eublepharis macularius (Eublepharidae). Illumina sequence data were generated from seven insert libraries (ranging from 170 to 20 kb), representing a raw sequencing depth of 136X from 303 Gb of data, reduced to 84X and 187 Gb after filtering. The assembled genome of 2.02 Gb was close to the 2.23 Gb estimated by k-mer analysis. Scaffold and contig N50 sizes of 664 and 20 kb, respectively, were comparable to the previously published Gekko japonicus genome. Repetitive elements accounted for 42 % of the genome. Gene annotation yielded 24,755 protein-coding genes, of which 93 % were functionally annotated. CEGMA and BUSCO assessment showed that our assembly captured 91 % (225 of 248) of the core eukaryotic genes, and 76 % of vertebrate universal single-copy orthologs.ConclusionsAssembly of the leopard gecko genome provides a valuable resource for future comparative genomic studies of geckos and other squamate reptiles.
| S-EPMC5080775 | biostudies-literature
Project description:Cryptosporidiosis is observed in reptiles with high morbidity and considerable mortality. The objective of this study was to achieve the molecular identification of Cryptosporidium spp. in pet leopard geckos (Eublepharis macularius) from a breeder colony in Buenos Aires, Argentina. Oocysts comparable to those of Cryptosporidium spp. were detected in three geckos with a history of diarrhea, anorexia and cachexia. Molecular identification methods confirmed the presence of Cryptosporidium varanii (syn. C. saurophilum). This agent was considered to be the primary cause of the observed clinical disease. This is the first description of C. varanii infection in pet reptiles in Argentina.
| S-EPMC4935766 | biostudies-literature