Actitis macularius
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PROVIDER: PRJNA882932 | ENA |
REPOSITORIES: ENA
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Project description:Eublepharis macularius Genome sequencing and assembly
| PRJNA339626 | ENA
Project description:Eublepharis macularius transcriptome to identify immunoglobulins
| PRJNA308941 | ENA
Project description:RNA-Seq from liver of Eublepharis macularius
| PRJDB3879 | ENA
Project description:A revised leopard gecko (Eublepharis macularius) reference genome
| PRJNA884264 | ENA
Project description:BackgroundGeckos are among the most species-rich reptile groups and the sister clade to all other lizards and snakes. Geckos possess a suite of distinctive characteristics, including adhesive digits, nocturnal activity, hard, calcareous eggshells, and a lack of eyelids. However, one gecko clade, the Eublepharidae, appears to be the exception to most of these 'rules' and lacks adhesive toe pads, has eyelids, and lays eggs with soft, leathery eggshells. These differences make eublepharids an important component of any investigation into the underlying genomic innovations contributing to the distinctive phenotypes in 'typical' geckos.FindingsWe report high-depth genome sequencing, assembly, and annotation for a male leopard gecko, Eublepharis macularius (Eublepharidae). Illumina sequence data were generated from seven insert libraries (ranging from 170 to 20 kb), representing a raw sequencing depth of 136X from 303 Gb of data, reduced to 84X and 187 Gb after filtering. The assembled genome of 2.02 Gb was close to the 2.23 Gb estimated by k-mer analysis. Scaffold and contig N50 sizes of 664 and 20 kb, respectively, were comparable to the previously published Gekko japonicus genome. Repetitive elements accounted for 42 % of the genome. Gene annotation yielded 24,755 protein-coding genes, of which 93 % were functionally annotated. CEGMA and BUSCO assessment showed that our assembly captured 91 % (225 of 248) of the core eukaryotic genes, and 76 % of vertebrate universal single-copy orthologs.ConclusionsAssembly of the leopard gecko genome provides a valuable resource for future comparative genomic studies of geckos and other squamate reptiles.
| S-EPMC5080775 | biostudies-literature
Project description:Cryptosporidiosis is observed in reptiles with high morbidity and considerable mortality. The objective of this study was to achieve the molecular identification of Cryptosporidium spp. in pet leopard geckos (Eublepharis macularius) from a breeder colony in Buenos Aires, Argentina. Oocysts comparable to those of Cryptosporidium spp. were detected in three geckos with a history of diarrhea, anorexia and cachexia. Molecular identification methods confirmed the presence of Cryptosporidium varanii (syn. C. saurophilum). This agent was considered to be the primary cause of the observed clinical disease. This is the first description of C. varanii infection in pet reptiles in Argentina.
| S-EPMC4935766 | biostudies-literature
Project description:Simple Summary Three adult female leopard geckos (Eublepharis macularius) belonging to three different owners were referred to for coelomic distention, anorexia, and weight loss. X-rays showed the presence of a macrosomic egg set in the third caudal of the coelomic cavity, and the diagnosis of dystocia was made in all three geckos. A cloacal endoscopic examination was performed on all three animals. A voluminous egg protruding through the urogenital papilla to the cloaca was visible. All the eggs were removed easily using endoscopic forceps. In two geckos, the eggshell was torn, and the content was aspirated to reduce the egg volume. After the procedure, a subcutaneous deslorelin implant was implanted. All geckos recovered rapidly after surgery. Two of the three geckos were healthy at the follow-up visit (respectively one and two years after the surgery) and did not show any signs of dystocia. Only in the third gecko, the dystocia recurred again 6 months later, and bilateral ovariosalpingectomy was performed. After surgery, the gecko recovered rapidly, resumed eating, and was discharged after one week of hospitalization in good condition. Abstract Dystocia is a multifactorial, life-threatening condition commonly affecting pet reptiles. Treatment for dystocia can be either medical or surgical. Medical treatment usually involves the administration of oxytocin, but in some species or, in some cases, this treatment does not work as expected. Surgical treatments such as ovariectomy or ovariosalpingectomy are resolutive, but invasive in small-sized reptiles. In this paper, we describe three cases of post ovulatory egg retention in three leopard geckos (Eublepharis macularius) successfully treated through a cloacoscopic removal of the retained eggs, after a non resolutive medical treatment. The intervention was fast, non-invasive, and no procedure-related adverse effects were noted. The problem relapsed six months later in one animal, and a successful bilateral ovariosalpingectomy was performed. Cloacoscopy should be considered a valuable, non-invasive tool for egg removal in dystocic leopard geckos when the egg is accessible to manipulation. Recrudescence or complications such as adhesions, oviductal rupture, or the presence of ectopic eggs should recommend surgical intervention.
| S-EPMC10000207 | biostudies-literature