Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici
Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici A total of 12 samples were analyzed, including three biological replicates of the four conditions.
Project description:Bacterial antimicrobial compounds and global regulatory networks are typically studied as separate systems, limiting our understanding of how these functions might be integrated. Here we reveal a dual-function system in the biocontrol strain Pseudomonas sp. MUP55, where the pvf cluster simultaneously functions as a global regulator of specialized metabolism and produces direct antimicrobial compounds. Metabolomic and transcriptomic analysis of a ∆pvfC mutant showed extensive global change of the regulation of metabolites and gene expression, with effects on specialized metabolites. Remarkably, pvfC differentially regulates dual siderophore systems and uncouples typically co-regulated small regulatory RNAs in the Gac/Rsm cascade. Heterologous expression confirmed the pvf cluster produces compounds with direct antimicrobial activity independent of its regulatory functions. Comparative genomic analysis revealed the MUP55 pvf cluster contains a rare additional pvfE gene found in only 3.4% of identified pvf clusters. This evolutionary integration of regulatory and defensive functions within a single genetic system provides an efficient strategy for bacterial competitive fitness and resource allocation, expanding our understanding of how beneficial microbes coordinate cellular processes while maintaining environmental competitiveness