Project description:To elucidate the molecular mechanism underlying lifespan reduction induced by PM2.5 exposure in Caenorhabditis elegans, we performed global gene expression profiling by RNA-sequencing technology, and compared the gene expression pattern change induced by PM2.5 exposure.
Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling
Project description:To unravel changes in gene expression due to exposure to PM2.5, we performed bulk RNA-seq analyses of zebrafish larvae exposed to PM2.5 and controls. Among others, PM2.5 increased oxoglutarate alpha-ketoglutarate receptor 1a, nitric oxide synthase, arachidonate 5-lipoxygenase b, immunity-related GTPase family e1, sulfotransferase family 5A, and macrophage expressed 1. NADPH oxidase organizer 1a and NADPH oxidase 1 were upregulated due to PM2.5 exposure. Furthermore, protein tyrosine/serine/threonine phosphatase activity was decreased after exposure to PM2.5. GESA analysis showed that genes involved in proteasome complex formation, inflammatory and immune response, leucocyte-mediated cytotoxicity, peptidase activator activity protein folding, and apoptotic signaling were upregulated after exposure to PM2.5. These results indicate that PM2.5 exposure caused the activation of immune-inflammatory and oxidative stress pathways and lipid and metabolic dysregulation.
Project description:Numerous epidemiological studies have demonstrated a significant association between PM2.5 exposure and pulmonary diseases. PM2.5 may contribute to epithelial injury and immune dysregulation in lung cells. In this study, we provide single-cell level gene expression profiles of mouse lungs under PM2.5 exposure, offering insights into the microenvironmental alterations and physiological responses induced by PM2.5.
Project description:The C. elegans lifespan in the presence of Bacillus licheniformis caused induction of a large number of genes associated with anti-aging activiy including beta-oxidation Inaddition, these results indicate the B. licheniformis enhances the lifespan of Caenorhabditis elegans through serotonin signaling Two-condition experiment, C. elegans with B. lichemiformis 141 or E. coli OP50 (conrol) for 24 h. For preparing the total RNA, C. elegans were exposed to 20 mg of bacterial lawn in NGN agar for 24 h.
Project description:Genome-wide analysis of lncRNA expression profiles in COPD rat model exposed by cigarette smoking (CS) and fine particulate matter (PM2.5). Goal was to explore the differences and similarities lncRNAs expression in rats model of COPD exposed by CS and PM2.5.
Project description:The animals were divided into two groups according to exposure of PM2.5 (particulate matter less than 2.5 µm): control group (Non-PM group) or PM exposure group (PM group).PM2.5 at 150 μg/m3were administered by intratracheal instillation during the gestation period of female rats from Day 1 of conception onwards.After the female rat gave birth,the offspring's heart DNA was extracted and anlyzed. DNA promotor methylation profile were determined.The goal was to determine the effects of PM2.5 exposure on the DNA promotor methylation patterns in heart tissue.
Project description:Exposure to PM2.5 affects gene expression of BEAS-2B cells Gene expression microarrays were used to detail changes in the transcriptome upon exposure to PM2.5