Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell gene expression profiles for isogenic PTEN panel iPSC-derived cortical organoids

ABSTRACT: Single cell gene expression profiles for isogenic PTEN panel iPSC-derived cortical organoids

PROVIDER: PRJNA885309 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR21751032.fastq.gz	Fastqsanger.gz
	SRR21751033.fastq.gz	Fastqsanger.gz
	SRR21751034.fastq.gz	Fastqsanger.gz
	SRR21751035.fastq.gz	Fastqsanger.gz
	SRR21751036.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Single cell gene expression profiles for isogenic PTEN panel iPSC-derived cortical organoids

Project description:Alterations in cortical neurogenesis are implicated in neurodevelopmental disorders including autism spectrum disorders (ASDs). Many ASD risk genes have been identified as critical for brain development, but the contribution of genetic backgrounds, although inferred in complex genetic disorders such as ASD, remains unclear. Here, using isogenic induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs) and cortical organoid models, we report that a heterozygous PTEN p.I135L mutation found in an ASD patient with macrocephaly dysregulates cortical neurogenesis in an ASD genetic background-dependent fashion. We found that this PTEN p.I135L mutation led to overproduction of NPC subtypes as well as neuronal subtypes including both deep and upper layer neurons in its ASD background, but not when introduced into a control genetic background. ASD and control background differences in neurogenesis, neural development and synapse signaling were also observed. These findings provide experimental evidence that both a PTEN p.I135L mutation and ASD genetic background contribute to cellular features consistent with ASD associated with macrocephaly.

2023-03-20 | GSE214422 | GEO

Bulk RNA-seq on isogenic PTEN panel iPSC-derived neural progenitor cells to study the effect of ASD specific PTEN mutation and the effects of autism genetic background

2023-03-20 | GSE214323 | GEO

Single cell gene expression profiles for iPSC-derived cortical organoids

2023-03-20 | GSE221882 | GEO

Bulk RNA-seq on isogenic PTEN panel iPSC-derived neural progenitor cells to study the effect of ASD specific PTEN mutation and the effects of autism genetic background

Project description:Bulk RNA-seq on isogenic PTEN panel iPSC-derived neural progenitor cells to study the effect of ASD specific PTEN mutation and the effects of autism genetic background

| PRJNA884749 | ENA

Expression data from isogenic Pten WT or KO mouse T-ALLs treated with DBZ or DMSO

Project description:To investigate the underlying mechanisms mediating resistance to NOTCH inhibition in Pten-null T-ALL tumor cells we performed gene expression profiling of isogenic Pten-positive and Pten-deleted leukemia lymphoblasts after acute treatment with DBZ in vivo. This analysis revealed that, while direct NOTCH1 target genes (such as Hes1, Dtx1, PtcrA, HeyL and Notch3) are effectively downregulated in both Pten-positive and Pten-deleted tumors, genetic ablation of Pten elicits a global reversal of much of the transcriptional effects of NOTCH inhibition. We performed microarray gene expression analysis of GSI treatment in isogenic Pten KO or WT NOTCH1 induced leukemias

2015-08-31 | E-GEOD-71087 | biostudies-arrayexpress

Proteome profiling of an isogenic CAG allelic panel of Huntington disease hESCs and NPCs

Project description:Whole proteome profiling and quantification was performed on an isogenic Huntington disease (IsoHD) human embryonic stem cell (hESC) allelic panel. The IsoHD hESCs harbour 30, 45, 65 and 81 CAG repeats in the first exon of HTT. Whole proteome quantification was also performed on neural progenitor cells derived from the IsoHD hESC panel.

2019-02-14 | PXD006585 | JPOST Repository

Gene expression from human iPSC derived cortical neurons

Project description:Gene expression from icas9 human iPSC derived cortical neurons treated with and without doxycycline

| EGAS00001004782 | EGA

Bulk RNA-seq on CTNNB1 mutation CRISPR-corrected ASD iPSC-derived neural progenitor cells as well as one matched control iPSC-derived neural progenitor cells to study the effects of autism genetic background

2023-03-20 | GSE221923 | GEO

Expression data from isogenic Pten WT or KO mouse T-ALLs treated with DBZ or DMSO

2015-08-31 | GSE71087 | GEO

WGS of KCNQ2 R201C patient derived iPSC and its isogenic line

Project description:Using prime editing, we generated an isogenic line of an iPSC line of an individual with a pathogenic KCNQ2 R201C mutation. WGS on the mutant line and two isogenic clones was performed to identify potential off target effects

| EGAS00001007231 | EGA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data