Ontology highlight
ABSTRACT:
PROVIDER: PRJNA885608 | ENA |
REPOSITORIES: ENA
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Project description:Dietary phytochemicals are plant-derived bioactive compounds that can influence host physiology and gut microbial communities. However, the structural distinctiveness of phytochemicals and their modulation of host intestinal gene expression in a sex-dependent manner remain poorly understood. In this study, we used Drosophila melanogaster to investigate gut transcriptomic responses to four phytochemical-enriched diets, capsaicin, curcumin, resveratrol, and thymoquinone. Two-day-old mated female and male flies were exposed to control or phytochemical-supplemented diets for 10 days, after which gut tissues were dissected for bulk RNA sequencing. Transcriptomic analysis revealed that biological sex was a major source of variation in the gut, with female and male flies showing distinct expression profiles across dietary treatments. Phytochemical supplementation induced compound and sex-specific transcriptional responses. Curcumin produced the strongest transcriptional response, with females showing reduced expression of genes involved in carbohydrate digestion and trehalose metabolism, whereas males showed enrichment of xenobiotic detoxification pathways dominated by cytochrome P450 genes. Capsaicin also induced sex-dependent responses, including detoxification-associated transcriptional changes in females and increased expression of immune-related genes in males. These results indicate that dietary phytochemicals elicit sex-specific gut transcriptomic responses in Drosophila melanogaster and highlight biological sex as an important variable in studies of diet-host interactions.
2026-05-11 | GSE330166 | GEO
Project description:Capsaicin has previously been demonstrated to exhibit anti-tumor effect in various cancer type. However, the deep biological function and molecular mechanism of capsaicin was still uncertain. In this research, we used high-throughput RNA sequencing to unveil the potential biological function of capsaicin in human gastric cancer cell line AGS.Total RNA was collected from AGS cells treated with capsaicin (at a dose of 250uM for 24h) or DMSO using TRIzol reagent according to the manufacturer’s protocol (n=3 per group). RNA was quantified using a NanoDrop ND-2000 (Thermo Scientific, USA), and RNA integrity was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, USA). High throughput sequencing was performed by TsingKe biotech Co., Ltd. according to the manufacturers` standard protocols. The quality control and preliminary analysis of sequencing raw data was performed by Novogene biotech Co., Ltd. according to the standard pipeline. Gene expression level was measured by Fragments Per Kilobase of exon model per Million mapped fragments (FPKM).
2024-11-01 | E-MTAB-14511 | biostudies-arrayexpress
Project description:We tried two methods which are the DNase I treated full-length double-strand cDNA sequencing and the poly(A) capture full-length double-strand cDNA sequencing to avoid the non-specific genomic DNA amplification.
2022-06-29 | GSE192928 | GEO
Project description:To uncoverthe capsaicin effects on the neutrophil activation, we performed transcriptomic of dHL-60 cells after capsaicin stimulation in degree of different time. this study revealed the functional changes of neutrophils in the presence of capsaicin. The transcriptomic data with different time stimulation gradients can contribute to the revealing of dynamic alternation in gene expression and annotate the time-dependent effect of the neutrophil respond to capsaicin.
2022-06-30 | GSE198304 | GEO
Project description:Type 2 diabetes (T2D), the most common form of diabetes, is one of the leading causes of death in the USA. Diet, genetics, environment, and the gut microbes are important factors causing T2D. However, the exactmechanisms by which these factors induce the disease are still unclear. Western diet-induced obesity mimicked the conditions of metabolic syndrome and T2D in mice. Concurrent changes in the bacterial abundance and composition in ileum and stool of mice indicated that the metabolic alterations are associated with microbes.Using a transkingdom network and causal inference analysis, we identified candidate microbes that can potentially mediate changes in metabolic health. In line with our predictions, supplementing mice with the beneficial microbial candidates ameliorated diet induced diabetes in mice. Transcriptomic studies showed that these microbes improved metabolic parameters by regulating mitochondrial processes in the liver. Using systems biology network analyses, followed by experimental validation, we identified key microbes and pathways regulating glucose metabolism. This research aims to uncover the mechanisms of microbiota dependent glucose metabolism to advance treatment of T2D.
2020-09-13 | GSE136033 | GEO
Project description:It has now been established that consumption of spicy food containing capsaicin is strongly associated with recurrence and worsening of symptoms in IBD.To uncover the potential signaling pathway involved in the capsaicin-induced relapse.To uncover the potential signaling pathway involved in the capsaicin-induced relapse,we performed proteomics of chronic colitis mice following capsaicin administration.Differential expression proteins which were tightly associated with inflammatory pathways have to be revealed.Differential expression proteins which were tightly associated with inflammatory pathways have to be revealed.The proteomic profile can help to identify the crucial pro-inflammatory factor implicated in the procedure of capsaicin-induced disease relapse.
2022-06-03 | PXD032186 | Pride