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Effect of ICSI on gene expression in germline stem cells

ABSTRACT: Effect of ICSI on gene expression in germline stem cells

PROVIDER: PRJNA886622 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21784964.fastq.gz	Fastqsanger.gz
	SRR21784965.fastq.gz	Fastqsanger.gz
	SRR21784966.fastq.gz	Fastqsanger.gz
	SRR21784967.fastq.gz	Fastqsanger.gz
	SRR25918343.fastq.gz	Fastqsanger.gz

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Effect of ICSI on gene expression in germline stem cells

Project description:To investigate the effect of ICSI in germ cells, we performed RNA-seq of germline stem cells established from ICSI-derived mice.

2023-09-21 | GSE214649 | GEO

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Project description:To investigate the effect of ICSI in germ cells, we performed RRBS of germline stem cells established from ICSI-derived mice.

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Effect of ICSI on DNA methylation in germline stem cells.

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Effect of intra-cytoplasmic sperm injection (ICSI) on gene expression and development of mouse preimplantation embryos

Project description:Background: In vitro culture of preimplantation mouse embryos is associated with changes in gene expression. It is however not known if the method of fertilization affects the global pattern of gene expression. Method: We compared gene expression and development of mouse blastocysts produced by intra-cytoplasmic sperm injection and cultured in Whitten’s medium (ICSIWM) or KSOM medium with amino acids (ICSIKSOMaa) with control blastocysts flushed out of the uterus on post coital day 3.5 (In vivo). Global pattern of gene expression was assessed using the Affymetrix 430 2.0 chip. In addition we compared gene expression in embryos generated in IVF or ICSI using WM. Results: Blastocysts resulting from ICSI fertilization have a reduction in the number of trophoblastic and inner cell mass cells compared to in vivo generated embryos. Approximately 1000 genes are different between ICSI blastocyst and in vivo blastocysts; proliferation, apoptosis and morphogenetic pathways are the most common pathways altered after in vitro culture. Unexpectedly, only 41 genes were statistically different between embryo cultured in suboptimal conditions (WM) or optimal conditions (KSOMaa). Conclusion: The method of fertilization plays a more important role in shaping the transcriptome of the developing mouse embryo than the culture media used.

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Regulation of germline gene expression in C. elegans germline stem cells.

Project description:ChIP-Seq study to understand regulation of germline stem cell gene expression by immunoprecipitation of stem-cell factor Notch and associated transcriptional cofactor LAG-1 in C. elegans.

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Effect of intra-cytoplasmic sperm injection (ICSI) on gene expression and development of mouse preimplantation embryos

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Differential Gene Expression of Human Cumulus Granulosa and Mural Granulosa Cells in ICSI Patients

Project description:Differential Gene Expression of Human Cumulus Granulosa and Mural Granulosa Cells in ICSI Patients

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Sequencing of proteomic pan-β-hydroxybutyrylation in female germline stem cells

Project description:Metformin increase the number of FGSCs in mouse ovary, and the increased pan-β-hydroxylbutylated protein modification level in vitro FGSCs was detected by WB. There are the Mass spectrum original data of proteomic examination about pan-β-hydroxybutyrylation sites in female germline stem cells after merformin treatment

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mRNA expression data from monkey PG-haESCs, ICSI-derived ESCs and somatic fibroblasts

Project description:Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes. Here,we report the derivation of haESCs from monkey parthenogenic blastocysts. These cells, which we designated PG-haESCs (parthenogenic haploid embryonic stem cells), express classical ESC markers, are pluripotent, and can differentiate to different cell lines from all three embryonic germ layers in vivo and in vitro. We used microarrays to compare the gene expression levels among PG-haESC, ICSI-derived ESCs and female monkey somatic fibroblasts. We used ICSI-derived ESCs and somatic fibroblasts isloated from female individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix Rhesus Macaque array.

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Transcriptomic profiling in equine IVF and ICSI embryos

Project description:In vitro production of equine embryos has been performed using intracytoplasmic sperm injection (ICSI) for the last two decades. Since 2022, a repeatable protocol for conventional in vitro fertilization (IVF) provides a successful alternative. However, little is known about the influence of the fertilization method on embryo quality and the transcriptomic profile. In this study, we aimed to examine differentially expressed genes (DEGs) between ICSI and IVF embryos in the horse. Therefore, ten equine sibling blastocysts, produced in vitro by either ICSI or IVF from three different mares, were subjected to Full-Length Single-Cell RNA-Sequencing (FLASH-sequencing). As such, 11,518 genes were identified, with only 1 DEG between ICSI and IVF embryos. Interleukin 4 induced 1 (IL4I1), a protein coding gene related to sperm cell death, was upregulated in IVF embryos (adjusted P-value = 0.01, FC = 2.84). Cleavage rates, calculated on collected COCs, of IVF zygotes (55.0%) were similar to those of ICSI zygotes (51.9%; P = 0.74), but blastocyst rates were higher following ICSI (37.0% vs 22.5% calculated on collected COCs and 71.4% vs 40.9% calculated on cleaved zygotes; P = 0.04 and 0.004, respectively). The average day of blastocyst development did not differ (P = 0.55). In conclusion, gene expression was similar for the two fertilization techniques, aside from one sperm related DEG, supporting the safety of equine IVF for further clinical studies. Overall, the horse provides a valuable model to study long-term effects of assisted reproductive technologies with potential extrapolation to human medicine. Keywords: Transcriptome, FLASH-seq, embryo, horse, IVP, fertilization

2025-11-26 | GSE308657 | GEO

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