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Mus musculus strain:C57BL/6N | isolate:Liver

ABSTRACT: Mus musculus strain:C57BL/6N | isolate:Liver Transcriptome or Gene expression

PROVIDER: PRJNA886862 | ENA |

REPOSITORIES: ENA

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Tunicamycin induced ER stress in liver of C57Bl/6N mice

Project description:ER stress could affect many tissues, especially liver, in which non-alcoholic fatty liver disease, liver steatosis, etc. have been reported relative. But there still lack systematic insight into ER stress in liver, which can be obtained by transcriptomics of the tissue. Here, tunicamycin was utilized to induce ER stress in C57BL/6N mice. And microarray was performed to get the transcriptome alteration. Microarray of liver from tunicamycin-injected C57Bl/6N mice

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Mus musculus domesticus strain:C57BL/6N

Project description:Mus musculus domesticus strain:C57BL/6N Transcriptome or Gene expression

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Project description:To investigate the effect of aging on mitochondrial gene expression we isolated liver mitochondria from 4 12-week and 4 65-week C57BL/6N WT mice. We then isolated RNA and prepared the barcoded library using PCR-cDNA Barcoding Kit SQK-PCB109 and ran it on MinIOn R9.4.1 Flow cells.

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