Ontology highlight
ABSTRACT:
PROVIDER: PRJNA887557 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR21820803_1.fastq.gz | Fastqsanger.gz | |||
| SRR21820803_2.fastq.gz | Fastqsanger.gz |
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Project description:Plastic pollution is a pressing global issue, with polyethylene (PE) the most widespread and persistent contaminant. Galleria mellonella (G. mellonella) has been identified as one of the most suitable insect species for the efficient consumption and degradation of PE; However, the gut microbiota and endogenous factors of G. mellonella contribute to efficient degradation of PE remain unclear. Here our metagenomic analyses revealed that the gut microbial diversity of larvae fed low-density polyethylene (LDPE) remained stable and showed no significant difference from that of the control group, indicating limited community restructuring during LDPE digestion. Proteomic and metabolomic profiling revealed elevated expression of redox-related proteins, accumulation of LDPE oxidative products, and a substantial amount of short-chain fatty acids that could be utilized by G. mellonella via metabolic pathways such as the TCA cycle. Strikingly, the oxidoreductase (luciferin 4-monooxygenase) consistently emerged as the most significantly differentially expressed protein in comparisons of LDPE-fed larvae against both the initial control and the beeswax groups, and it was predicted to exhibit strong binding affinity for long-chain alkenes. A key gut microbe, Brevibacillus parabrevis strain B3, exhibited the highest activity in LDPE degradation. Importantly, in vitro assays demonstrated that the combination of luciferin 4-monooxygenase and Brevibacillus parabrevis strain B3 synergistically enhanced LDPE degradation efficiency-far surpassing enzyme or bacterial treatments alone. Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed significant oxidative surface modifications, including hydroxyl and carbonyl group formation, under combined treatment. These results suggest that Gm-luciferin 4-monooxygenase likely acts as the principal driver of LDPE degradation in G. mellonella, with other oxidoreductases and gut bacteria providing auxiliary support. Our findings elucidate the enzymatic and microbial synergy underlying wax worm-mediated LDPE biodegradation and offer promising targets for developing bio-inspired plastic waste remediation technologies.
2026-04-11 | MTBLS14246 | MetaboLights
Project description:The Galleria mellonella larvae were infected with Listeria monocytogenes and on the 5th of post infection RNA is isolated from infected and non-infected control larvae. RNA samples were processed for miRNA profile in response to L. monocytogenes infection in Galleria mellonella larvae.
2017-10-30 | E-MTAB-4024 | biostudies-arrayexpress
Project description:Galleria mellonella larvae efficiently biodegrade LDPE plastic via gut monooxygenase activity.
2026-02-06 | PXD074229 |
Project description:Plastics are highly stable materials with widespread applications, but their resistance to degradation poses a significant environmental challenge, often resulting in accumulation in landfills or pollution in the form of microplastics. Biodegradation using insect larvae has recently emerged as a promising strategy to address this issue, though the molecular basis of plastic degradation in these organisms remains poorly understood due to limited genomic resources. In this study, we present a complete genome of the lesser wax moth, Achroia grisella, and tissue-specific RNA-Seq data of both the lesser and the greater wax moth, Galleria mellonella, two species known to consume various plastics. Our analyses reveal several highly expressed secretory enzymes in gut and labial tissues. Orthologous comparisons of differentially expressed genes also identified five enzymes (three hexamerins and two monooxygenases) from the lesser wax moth that have been shown or are predicted to have plastic-degrading potential in the greater wax moth. We also identified enzymes that may potentially be involved in polyethylene and polystyrene degradation based on their identities with known bacterial enzymes that have been experimentally validated and are involved in plastic degradation pathways. Together, these genomic and transcriptomic resources provide a foundation for understanding plastic degradation in wax moths and highlight candidate genes for future functional validation.
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Project description:Galleria mellonella genome
| PRJNA893711 | ENA