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Genome-scale CRISPR screening in a single mouse liver

ABSTRACT: Genome-scale CRISPR screening in a single mouse liver

PROVIDER: PRJNA889363 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21859340.fastq.gz	Fastqsanger.gz
	SRR21859341.fastq.gz	Fastqsanger.gz
	SRR21859342.fastq.gz	Fastqsanger.gz
	SRR21859343.fastq.gz	Fastqsanger.gz
	SRR21859344.fastq.gz	Fastqsanger.gz

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Genome-scale CRISPR screening in a single mouse liver

Project description:This paper describes the development and implementation of genome-scale CRISPR screening in the mouse liver. As part of this study, RNA sequencing was performed on livers isolated from mice during development, adulthood, and regeneration to identify the entirety of genes expressed in the liver at each time point.

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Genome-scale CRISPR screening in a single mouse liver.

Project description:A complete understanding of the genetic determinants underlying mammalian physiology and disease is limited by the capacity for high-throughput genetic dissection in the living organism. Genome-wide CRISPR screening is a powerful method for uncovering the genetic regulation of cellular processes, but the need to stably deliver single guide RNAs to millions of cells has largely restricted its implementation to ex vivo systems. There thus remains a need for accessible high-throughput functional genomics in vivo. Here, we establish genome-wide screening in the liver of a single mouse and use this approach to uncover regulation of hepatocyte fitness. We uncover pathways not identified in cell culture screens, underscoring the power of genetic dissection in the organism. The approach we developed is accessible, scalable, and adaptable to diverse phenotypes and applications. We have hereby established a foundation for high-throughput functional genomics in a living mammal, enabling comprehensive investigation of physiology and disease.

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Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage [CRISPR screening]

Project description:Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage [CRISPR screening]

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Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage

Project description:Overall data for CRISPR screening + RNA-Seq

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Project description:This is an in vitro genome-wide CRISPR/cas9 screen in human glioblastoma stem cells, screening for genes essential for survival of these cells. These cells express cas9 and have been transfected with a guide RNA library causing gene knockouts. We will analyse the sequencing data for depletion of guide RNAs. In this particular study, we will do RNA sequencing to correlate CRISPR with expression levels in specific cancer cell subpopulations. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

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Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage [CRISPR screening]

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Genome-scale CRISPR-Cas9 knockout screening for identification of genes which regulate CD38 expression in RPMI 8226 cells

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