Project description:We quantify the effects of electrical stimulation on murine cells at the cellular and transcriptomic level using single cell RNA-sequencing. We characterize circulating cells and compare them to unstimulated circulating cells.
Project description:Early diagnosis and timely intervention are crucial for the management of periodontal diseases driven by immune dysregulation. However, in situ sensing and drug delivery in the oral cavity remain challenging due to biological disparities between electronic devices and oral tissues. Here, we report a flexible, battery-free, and hydrogel-based bioelectronics that integrates biological functions and wireless signal transmission for periodontal inflammation monitoring and localized microRNA (miRNA) immunomodulation. The hydrogel, synthesized from gelatin with bioactive peptide sequences cleavable by matrix metalloproteinases (MMPs), responds specifically to protease activity associated with periodontal disease progression. Its enzymatic degradation is wirelessly transduced into quantitative radio-frequency signals of local immune activity, while simultaneously triggering the release of embedded miRNAs as critical immune regulators. In vitro and animal validations demonstrated diagnostic accuracy and therapeutic efficacy, leading to suppression of the inflammatory response. The combination of biosensing, wireless feedback, and miRNA-based immunotherapy provides a closed-loop, adaptable platform for personalized management of periodontal and other inflammation-related disorders.
Project description:The aim of the study is to identify a pattern of chemoresistive sensors able to recognise the presence of a tumoral pathology from a health state through the analysis of Volatile Organic Compounds inside the specimen.
The chemoresistive nanostructured sensors are into an innovative patented device SCENT B1 which can analyse different specimens: blood samples, tissue biopsies, cell cultures.
In this study SCENT B1 wil be used to compare the measures of:
* tumoral and health tissues taken from different neoplasms after their surgical resection
* blood samples from healthy and tumor affected people
* pre and post- operative blood samples of tumor affected people
Project description:Ezrin is a cytoplasmic protein that can exist in multiple conformational forms that are regulated by phosphorylation at the threonine 567 residue. Phosphorylated open form of ezrin was generally accepted as the active form because it can translocate to the plasma membrane whereas unphosphorylated closed ezrin, sequestered in the cytoplasm, have been considered inactive. Here, we identified closed ezrin as a novel RNA binding protein with biological activity. Our previous work established that ezrin directly interacts with cytoplasmic RNA binding proteins (RBPs). Here we show that ezrin expression correlates with RBPs in human osteosarcoma samples. Recombinant ezrin in its closed conformation directly bound RNA with 6.1 nM affinity. Ezrin binding specificity was highest for the guanine-rich sequences and RNA G-quadruplexes (G4 RNA). Reintroduction of closed ezrin expression rescued the transcriptome and proteome profiles in an ezrin-null osteosarcoma cell line. Closed ezrin also bound endogenous mRNAs that were enriched for pathways related to RNA processing and splicing, DNA maintenance, and cellular metabolism. Closed ezrin also rescued the metastatic capability of osteosarcoma xenografts in zebrafish. Taken together, our data established that the closed conformation of ezrin, previously thought to be inactive, can directly bind RNA and contribute to the metastatic phenotype.