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ABSTRACT: Integrated Metabolome and Transcriptome Profiling Provide Insights into Cold Responses in Rapeseed (Brassica napus L.) Genotypes with Contrasting Cold Stress Sensitivity

PROVIDER: PRJNA890757 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21919087_1.fastq.gz	Fastqsanger.gz
	SRR21919087_2.fastq.gz	Fastqsanger.gz
	SRR21919088_1.fastq.gz	Fastqsanger.gz
	SRR21919088_2.fastq.gz	Fastqsanger.gz
	SRR21919089_1.fastq.gz	Fastqsanger.gz

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Project description:Oil rapeseed (Brassica napus L.) is a typical winter biennial plant, with high cold tolerance during vegetative stage. In recent years, more and more early-maturing rapeseed varieties were planted across China. Unfortunately, the early-maturing rapeseed varieties with low cold tolerance have higher risk of freeze injury in cold winter and spring. Little is known about the molecular mechanisms for coping with different low-temperature stress conditions in rapeseed. In this study, we investigated 47,328 differentially expressed genes (DEGs) of two early-maturing rapeseed varieties with different cold tolerance treated with cold shock at chilling (4°C) and freezing (−4°C) temperatures, as well as chilling and freezing stress following cold acclimation or control conditions. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that two conserved (the primary metabolism and plant hormone signal transduction) and two novel (plant-pathogen interaction pathway and circadian rhythms pathway) signaling pathways were significantly enriched with differentially-expressed transcripts. Our results provided a foundation for understanding the low-temperature stress response mechanisms of rapeseed. We also propose new ideas and candidate genes for genetic improvement of rapeseed tolerance to cold stresses.

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