Project description:Streptococcus agalactia were isolated from virginal swabs and from bovine. the experiment aims at differentiating the proteome and metabolome differences between the 2 isolated strains. To achieve this target, label free proteomics and untargeted metabolomics profiling were applied
Project description:Streptococcus suis is an important zoonotic pathogen that can cause meningitis and sepsis in both pigs and humans. In this study,we evaluated the genetic difference of 40 Streptococcus suis strains belonging to various sequence types by comparative genomic hybridization to identify genes associated with the variation in pathogenicity using NimbleGen’s tilling microarray platform. Application of Comparative Phylogenomics to Identify Genetic Differences Relating to Pathogenicity of Streptococcus suis
Project description:Streptococcus suis 2 Rgg-dependent transcription was analyzed. Microarray analysis was performed using RNA samples isolated from Streptococcus suis 2 wild-type strain 05ZYH33 as well as RNA isolated from 05ZYH33 rgg isogenic mutant strain during postexponential phases of growth.
Project description:Streptococcus suis is an important zoonosis pathogen that causes significant economic losses worldwide characterized by meningitis, septicaemia, arthritis, bronchopneumonia endocarditis. Streptcoccus suis 2 strain SC19 was isolated in Sichuan province in China, during the outbreak in 2005. Septicemia is most popular symptoms for SC19 infection, and mortality is high. We used human acute monocytic leukemia cell line (THP-1) infected SC19 to analysis the pathomechanism of septicemia in SS2 infection.
Project description:Febrile patients PCR positive for H1N1 swine flu, seasonal H1N1 and seasonal H3N2 in nasal swabs and controls consisting of febrile patients with rhinovirus infection or febrile patients of non-viral etiology (nasal swabs PCR negative for common respiratory viruses and blood PCR negative for dengue and parvovirus B19) were assessed consecutively for global transcriptional changes in whole blood
Project description:We used microarrays to detail the global gene expression changes following apical infection of porcine choroid plexus epithelial cells (PCPEC) with Streptococcus suis (S. suis)
Project description:There is a paucity of data on how the mucosal immune system changes with age. Moreover, the link between nasal presence of bacteria and viruses with the immune system in young children is still poorly understood. To address these questions we collected nasal curettes, nasal swabs and filter papers from young children undergoing anesthesia for surgery unrelated to respiratory complaints. Microbial and host parameters were analysed from these samples, including transcriptomics from nasal curettes and swabs. Results revealed strong differences in nasal mucosal immunity in children compared with adutls, as well as clear effects of the presence of bacteria such as pneumococcus and haemophilus on the local immune system