Project description:This project contains single-cell RNA sequencing (scRNA-seq) data of the ovarian stroma from the viviparous teleost, black rockfish (Sebastes schlegelii). To define how the ovarian stroma is reprogrammed across reproduction, we profiled stromal cells spanning three distinct biological states: unfertilized, normal-gestation, and abnormal-gestation (characterized by spontaneous embryonic mortality and avascularity). This dataset aims to characterize the stromal cell composition, developmental trajectories, and immune-vascular interactions critical for maintaining intraovarian gestation, providing insights into the evolutionary biology of teleost viviparity.
Project description:Whether in aquaculture or in nature, starvation stress limits the growth of fish. The purpose of the study was to clarify the detailed molecular mechanisms underlying starvation stress in Korean rockfish (Sebastes schlegelii) through liver transcriptome and metabolome analysis. Transcriptome results showed that liver genes associated with cell cycle and fatty acid synthesis were down-regulated, whereas those related to fatty acid decomposition were up-regulated in the experimental group (EG; starved for 72 days) compared to the control group (CG; feeding). Metabolomic results showed that there were significant differences in the levels of metabolites related to nucleotide metabolism and energy metabolism, such as purine metabolism, histidine metabolism and oxidative phosphorylation. Five fatty acids (C22:6n-3; C22:5n-3; C20:5n-3; C20:4n-3; C18:3n-6) were selected as possible biomarkers of starvation stress from the differential metabolites of metabolome. Subsequently, correlation between these differential genes of lipid metabolism and cell cycle and differential metabolites were analyzed, and observed that these five fatty acids were significantly correlated with the differential genes. These results provide new clues for understanding the role of fatty acid metabolism and cell cycle in fish under starvation stress. It also provides a reference for promoting the biomarker identification of starvation stress and stress tolerance breeding research.
2023-01-24 | GSE223294 | GEO
Project description:Transcriptomic analysis of the response mechanisms of black rockfish (Sebastes schlegelii) under noise stress from offshore wind farms
| PRJNA1077754 | ENA
Project description:Sebastes schlegelii RNA-Seq data for Meox1 knockdown
