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Post-transcriptional regulation in cranial neural crest cells expands developmental potential [I]

ABSTRACT: Post-transcriptional regulation in cranial neural crest cells expands developmental potential [I]

PROVIDER: PRJNA891631 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21943866_1.fastq.gz	Fastqsanger.gz
	SRR21943866_2.fastq.gz	Fastqsanger.gz
	SRR21943867_1.fastq.gz	Fastqsanger.gz
	SRR21943867_2.fastq.gz	Fastqsanger.gz
	SRR21943868_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Post-transcriptional regulation in cranial neural crest cells expands developmental potential [II]

Project description:Developmental potential is progressively restricted after germ layer specification during gastrulation. However, cranial neural crest cells challenge this paradigm, as they develop from anterior ectoderm yet give rise to both mesodermal derivatives of the craniofacial skeleton and ectodermal derivatives of the peripheral nervous system. How cranial neural crest cells differentiate into multiple lineages is poorly understood. Here, we demonstrate that cranial neural crest cells possess a transient state of increased chromatin accessibility; and that the earliest premigratory neural crest are biased towards either a neuronal or ectomesenchymal fate, with each lineage expressing distinct factors from the pluripotent state. We profile the spatiotemporal emergence of each neural crest population and demonstrate that the ectomesenchymal lineage forms prior to the neuronal progenitors. Expression of the pluripotency microRNA family miR-302 is maintained in cranial neural crest cells and genetic deletion leads to precocious specification of the ectomesenchymal lineage. We find that miR-302 directly targets Sox9 to slow the timing of ectomesenchyme induction and regulates multiple genes involved in chromatin condensation to maintain accessibility for neuronal differentiation. Loss of mir-302 results in reduced chromatin accessibility in the neuronal progenitor lineage of neural crest and a reduction in peripheral neuron differentiation. Our findings reveal a post-transcriptional mechanism governed by miRNAs from pluripotency as an important mechanism to expand developmental potential of cranial neural crest.

2022-10-25 | GSE216136 | GEO

Post-transcriptional regulation in cranial neural crest cells expands developmental potential [I]

2022-10-25 | GSE215977 | GEO

Post-transcriptional regulation in cranial neural crest cells expands developmental potential

Project description:Post-transcriptional regulation in cranial neural crest cells expands developmental potential

| PRJNA892136 | ENA

Post-transcriptional regulation in cranial neural crest cells expands developmental potential [II]

Project description:Post-transcriptional regulation in cranial neural crest cells expands developmental potential [II]

| PRJNA892142 | ENA

Post-transcriptional regulation in cranial neural crest cells expands developmental potential.

Project description:Developmental potential is progressively restricted after germ layer specification during gastrulation. However, cranial neural crest cells challenge this paradigm, as they develop from anterior ectoderm, yet give rise to both ectodermal derivatives of the peripheral nervous system and ectomesenchymal bone and cartilage. How cranial neural crest cells differentiate into multiple lineages is poorly understood. Here, we demonstrate that cranial neural crest cells possess a transient state of increased chromatin accessibility. We profile the spatiotemporal emergence of premigratory neural crest and find evidence of lineage bias toward either a neuronal or ectomesenchymal fate, with each expressing distinct factors from earlier stages of development. We identify the miR-302 miRNA family to be highly expressed in cranial neural crest cells and genetic deletion leads to precocious specification of the ectomesenchymal lineage. Loss of mir-302 results in reduced chromatin accessibility in the neuronal progenitor lineage of neural crest and a reduction in peripheral neuron differentiation. Mechanistically, we find that mir-302 directly targets Sox9 to slow the timing of ectomesenchymal neural crest specification and represses multiple genes involved in chromatin condensation to promote accessibility required for neuronal differentiation. Our findings reveal a posttranscriptional mechanism governed by miRNAs to expand developmental potential of cranial neural crest.

| S-EPMC9963983 | biostudies-literature

Post-transcriptional regulation in cranial neural crest cells expands developmental potential

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-25 | GSE216138 | GEO

Identification of cranial-specific neural crest genes by comparative transcriptomics

Project description:We employ RNA-seq of FACS sorted cell populations to identify genes that are enriched in cranial neural crest in relationship to the trunk. Transcriptional profiling of delaminating cranial and trunk neural crest subpopulations.

2016-06-24 | E-GEOD-75125 | biostudies-arrayexpress

Single-cell profiling of cranial neural crest diversification across a vertebrate lifetime

Project description:The cranial neural crest (CNC) is a vertebrate-specific population that generates a huge diversity of derivatives, including the bulk of the connective and skeletal tissues of the head. How neural crest cells generate the appropriate cell types for distinct head regions over time remains unresolved. Here we profile RNA expression (scRNAseq) and chromatin accessibility (snATACseq) of the zebrafish cranial neural crest lineage at single-cell resolution from embryos to adults.

2021-11-11 | GSE178969 | GEO

Identification of cranial-specific neural crest genes by comparative transcriptomics

Project description:We employ RNA-seq of FACS sorted cell populations to identify genes that are enriched in cranial neural crest in relationship to the trunk.

2016-06-24 | GSE75125 | GEO

Gene bivalency at Polycomb domains regulates cranial neural crest positional identity [ATAC-seq]

Project description:The cranial neural crest cells are pluripotent cells that provide head skeletogenic mesenchyme and are crucial for craniofacial patterning. Here, we analyzed the in vivo chromatin landscapes of mouse cranial neural crest subpopulations. Early postmigratory neural crest subpopulations contributing to distinct mouse craniofacial structures displayed similar chromatin accessibility patterns, yet differed transcriptionally. Accessible promoters and enhancers of differentially silenced genes carried H3K27me3/H3K4me2 bivalent chromatin marks embedded in large Ezh2-dependent Polycomb domains, indicating transcriptional poising. These postmigratory bivalent regions were already present in neural crest premigratory progenitors. At Polycomb domains, H3K27me3 antagonized H3K4me2 deposition, which was restricted to accessible sites. Thus, bivalent Polycomb domains provide a chromatin template for the regulation of cranial neural crest cell positional identity in vivo contributing novel insights into the epigenetic regulation of face morphogenesis.

2017-03-31 | GSE89436 | GEO

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