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ABSTRACT: RNA-seq for chicken pre-hierarchical and hierarchical follicles granulosa cells

PROVIDER: PRJNA891942 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21968764_1.fastq.gz	Fastqsanger.gz
	SRR21968765_1.fastq.gz	Fastqsanger.gz
	SRR21968766_1.fastq.gz	Fastqsanger.gz
	SRR21968767_1.fastq.gz	Fastqsanger.gz
	SRR21968768_1.fastq.gz	Fastqsanger.gz

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Project description:RNA-seq for chicken pre-hierarchical follicles granulosa cells

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Quantitative proteomics of lipid droplets from goose hierarchical granulosa cells and pre-hierarchical granulosa cells

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2025-03-26 | PXD061272 | Pride

Single-cell RNA-seq uncovers cellular heterogeneity of granulosa cells and provides a signature for follicular development in chicken

Project description:The functional differentiation of the granulosa cell (GC) layer plays an essential role in follicular development and oocyte maturation in avian. In the current study, single-cell RNA sequencing (scRNA-seq) was employed to identify GC types, uncover heterogeneity, and construct developmental trajectories of chicken GCs in two developmental stages of hierarchical follicles (HF-GCs) and prehierarchical (PHF-GCs). The ﬁndings enhance our understanding of the cellular biology of GC layer of ovarian follicles‌ ‌and will assist studies of the molecular mechanisms of follicle development and selection in chicken.

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The potential functions of FOXL2 in different phases of chicken granulosa cells by RNA-seq

Project description:RNA-seq were performed to study the altered transcriptions by overexpression of FOXL2 in pre-hierarchical (SGCs) and pre-ovulatory granulosa cells (BGCs)

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Project description:Ovarian follicle development is a complex and well-orchestrated biological process. Granulosa cells (GCs) are the most important somatic cells in the ovary, which play important roles in the growth of follicles. Three-dimensional (3D) chromatin architecture has emerged as an important regulator of transcription in eukaryote, but how these changes in granulosa cells during folliculogenesis of chicken are largely unexplored. Here, we performed an integrative analysis of transcriptomic and chromatin structural characterization for chicken follicular granulosa cells of 10 and 3 growth stages respectively.

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Global comparative analysis on proteomes of chicken ovarian follicles before and after follicle selection

Project description:Ovarian follicle selection plays an important role in the reproduction of sexually mature hens, and this process can directly affect the growth and development of follicles until the final ovulation, thus affecting laying performance and fecundity of hens. In the laying hen ovary, one follicle from a cohort of 8-13 follicles of 6-8 mm in diameter is selected daily to enter the preovulatory hierarchy. In this study, we globally compared the proteomes of chicken ovarian follicles before and after follicle selection. A total of 5883 proteins were identified in the proteomes of chicken 6-8 mm prehierarchical follicles and 12-15 mm hierarchical follicles. 259 proteins are differentially expressed in 12-15 mm hierarchical follicle compared with prehierarchical follicle, of which 175 proteins are up-regulated and 84 proteins are down-regulated. The Gene Ontology enrichment of differentially expressed proteins revealed enriched GO terms for peptidase activity, acrosin binding for their molecular function and in the process of negative regulation of peptidase activity, and regulation of fertilization. The KEGG pathway analysis indicated that differentially expressed proteins were enriched for ribosome, lysine degradation, and endocytosis pathways. Nine differentially expressed proteins including vitellogenin-1 were validated with Parallel Reaction Monitoring (PRM) analysis, and their functions were discussed. This study provided a global proteomic view of the development of chicken ovarian follicles, which will serve as a foundation for understanding the molecular signatures and pathways of follicle selection in hens.

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Project description:Folliculogenesis in mammals is a cyclic and hierarchical process that the ovarian follicles undergo dramatic changes in the shape and number of granulosa cells accompanied by gradually enlarged immature oocyte. Here we show a thorough and high-resolution view of DNA methylation maps in oocyte free of somatic cells present at each distinct follicular development stages. We found that global DNA establishment occurs in Type5b follicles, the final stage of pre-antral follicles, and that the acquisition of DNA methylation at different trans-regulatory elements couples with different chromatin modifiers. Besides, we showed that Dnmt1 as well as Uhrf1 also play critical roles in requiring and retaining of de novo methylation statue, typically at LINE and LTR elements, in these immature oocytes, which successfully escape DNA replication. Our study thus refined the current knowledge on the establishment of unique DNA methylation profile in mammalian oocytes, and provide a valuable resource for further studies on female gametogenesis.

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