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Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

ABSTRACT: Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

PROVIDER: PRJNA892959 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22007161_1.fastq.gz	Fastqsanger.gz
	SRR22007161_2.fastq.gz	Fastqsanger.gz
	SRR22007162_1.fastq.gz	Fastqsanger.gz
	SRR22007162_2.fastq.gz	Fastqsanger.gz
	SRR22007163_1.fastq.gz	Fastqsanger.gz

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Assessment of the impact of the TLR3 L412F variant in terms of TLR3-sensitive transcript induction

Project description:we assessed the impact of TLR3 L412F at endogenous cellular levels upon double stranded RNA (dsRNA) stimulation, by performing RNA-sequencing in primary fibroblasts from individuals homozygous for the ancestral allele (n =2) or homozygous for TLR3 L412F (n = 4) upon extracellular Poly(I:C) stimulation that activates the TLR3 pathway. The average response of TLR3 L412F homozygotes was significantly decreased (mean Δ log2 fold-change = 0.93; Wilcoxon p <10-16) compared to that of cells with the ancestral allele, approaching that of TLR3-deficient cells, used as negative control.

2023-01-13 | GSE216304 | GEO

Assessment of the impact of the IL23R R381Q variant in terms of IL-23-sensitive transcript induction

Project description:Assessment of the impact of the IL23R R381Q variant in terms of IL-23-sensitive transcript induction

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Assessment of the impact of the IL23R R381Q variant in terms of IL-23-sensitive transcript induction

Project description:We stimulated T-cell blasts from IL23R R381Q homozygotes (n = 2), healthy controls homozygous for the ancestral allele (n = 9), homozygous individuals for TYK2 P1104A (n = 2) that selectively impairs responses to IL-23, and one patient each with autosomal recessive, complete TYK2 or IL-12Rb1 deficiency with IL-12, IL-23, IL-1b, and IL-1b plus IL-23 for 6 hours and performed RNA-sequencing. We used IL-1b as an IL-23-sensitizing agent as previously reported. We found that while T-cell blasts from IL23R R381Q homozygotes respond normally to IL-12 and IL-1b, their response to IL-23 and to IL-23 plus IL-1b is impaired

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Project description:The objective of this study is to study the Toll-like receptor 3 (TLR3)-dependent gene expression in human fibroblast cells and peripheral blood mononuclear cells (PBMCs) Fibroblast cells and PBMCs from donors with autosomal recessive (AR) complete TLR3 deficiency and autosomal dominant (AD) partial TLR3 deficiency were collected for microarray analysis to measure the transcriptional responsiveness of TLR3 in fibroblast and PBMCs. A total of 3 healthy controls, 1 AD TLR3-deficient patient, 1 AR TLR3-deficient patient, 1 AR UNC-93B-deficient patient, 1 MyD88 deficient patien were used in this study. The fibroblast cells were cultured in DMEM medium supplemented with 10% fetal calf serum (FCS). The PBMCs were cultured in RPMI medium complemented with 10% FCS. Different kinetics (2 hours and 8 hours) of poly(I:C) (25ug/ml, purchased from Amersham) stimulations were performed, aiming to define TLR3-inducible genes in these human cells. A stimulation of IL-1β (20ng/ml, purchased from R&D system Inc.) was used as a positive activation control with same kinetics of stimulations. A total of one million cells per stimulation were used for fibroblasts, and around 1.5 million cells per stimulation for PBMCs.

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Project description:In this study, we sequenced RNAs localized in the early endosome and lysosome and TLR3-binding RNAs, and identified Rmrp as a TLR3 binding early endosomal lncRNA. Further we investigated the biological funtion of Rmrp in vitro and in vivo, proving that Rmrp posivitely regulated TLR3 activation by inducing TLR3 dimerization. Cryo-EM structure of Rmrp-C-mTLR3-ECD complex further proved our conclusion.

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Endosomal lncRNA Rmrp is required for TLR3 innate sensing by engaging TLR3 dimerization

Project description:Endosomal lncRNA Rmrp is required for TLR3 innate sensing by engaging TLR3 dimerization

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Phosphorylated TLR3 nuclear translocation in cancer cell promotes metastasis and chemoresistance

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