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Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

ABSTRACT: Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

PROVIDER: PRJNA893367 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22016691_1.fastq.gz	Fastqsanger.gz
	SRR22016691_2.fastq.gz	Fastqsanger.gz
	SRR22016692_1.fastq.gz	Fastqsanger.gz
	SRR22016692_2.fastq.gz	Fastqsanger.gz
	SRR22016693_1.fastq.gz	Fastqsanger.gz

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Glis1 and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival

Project description:transcription factor Glis1 and metabolite oxaloacetic acid (OAA) could provide NP cells with survival tools for the harsh niche conditions in the IVD.

2022-10-28 | GSE216377 | GEO

<i>Glis1</i> and oxaloacetate in nucleus pulposus stromal cell somatic reprogramming and survival.

Project description:Regenerative medicine aims to repair degenerate tissue through cell refurbishment with minimally invasive procedures. Adipose tissue (FAT)-derived stem or stromal cells are a convenient autologous choice for many regenerative cell therapy approaches. The intervertebral disc (IVD) is a suitable target. Comprised of an inner nucleus pulposus (NP) and an outer annulus fibrosus (AF), the degeneration of the IVD through trauma or aging presents a substantial socio-economic burden worldwide. The avascular nature of the mature NP forces cells to reside in a unique environment with increased lactate levels, conditions that pose a challenge to cell-based therapies. We assessed adipose and IVD tissue-derived stromal cells through in vitro transcriptome analysis in 2D and 3D culture and suggested that the transcription factor Glis1 and metabolite oxaloacetic acid (OAA) could provide NP cells with survival tools for the harsh niche conditions in the IVD.

| S-EPMC9671658 | biostudies-literature

Expression data from synovium, bone marrow and nucleus pulposus

Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed. Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.

2010-10-11 | E-GEOD-24612 | biostudies-arrayexpress

MicroRNAs expression profiling of human nucleus pulposus cells: control vs. degeneration

Project description:MicroRNAs expression profiling of human nucleus pulposus cells derived from patients with disc degeneration in comparison with those derived from patients with scoliosis as control. Two-condition experiment: control nucleus pulposus cells vs. degenerative nucleus pulposus cells. Biological replicates: 3 control, 3 degenerated, independently harvested. Four replicates per array. The supplementary file 'GSE19943_fold_pvalue.txt' contains fold-changes and p-values.

2012-01-01 | E-GEOD-19943 | biostudies-arrayexpress

Expression data from bovine nucleus pulposus interverteral disc cells

Project description:Assessment of the putative differential gene expression profiles in high osmolality-treated bovine nucleus pulposus intervertebral disc cells for a short (5 h) and a long (24 h) time period. Identification of novel genes up- or down-regulated as an early or a late response to hyperosmotic stress. A 5 and 24 h-hyperosmotic treatment of nucleus pulposus cells led to transcriptional changes in >100 and 200 genes, respectively. Nucleus pulposus intervertebral disc cells were exposed to hyperosmotic stress for 5 and 24 h before RNA extraction and transcriptomics analysis. Three biological replicates were tested for each condition. Selected genes found to be differentially expressed were validated by RT-qPCR. Functional experiments were performed in order to assess the role of specific proteins encoded by genes found to be up-regulated in the osmo-reguatory response of intervertebral disc cells.

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human nucleus pulposus cell LC-MS/MS

Project description:To identify PPP1CA interactome in human nucleus pulposus cell

2023-10-24 | PXD042397 | Pride

human nucleus pulposus cell LC-MS/MS

Project description:To investigate whether SUV39H2 binds to PPP1CA in nucleus pulposus cells

2023-10-24 | PXD042152 | Pride

Expression data from synovium, bone marrow and nucleus pulposus

2010-10-11 | GSE24612 | GEO

Single cell sequencing of human nucleus pulposus

Project description:In order to discover the cell type in nucleus pulposus and find the cell type specific genetic change during intervertebral disc degeneration, we applied single cell RNA sequencing of nucleus pulposus tissue from degenerated and non-degenerated disc.

2023-12-31 | GSE153066 | GEO

A transcriptome analysis of serum-starved nucleus pulposus cells

Project description:This study sought to elucidate the transcriptomic changes of nucleus pulposus cells in serum starvation. The findings will lead to a better understanding of quiescent nucleus pulposus cells and provide insights into disc degeneration at the molecular level.

2025-05-01 | GSE232380 | GEO

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