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In vivo growth of subclones derived from Lewis lung carcinoma is determined by the tumor microenvironment not intrinsic proliferative ability

ABSTRACT: In vivo growth of subclones derived from Lewis lung carcinoma is determined by the tumor microenvironment not intrinsic proliferative ability

PROVIDER: PRJNA893542 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22020012.fastq.gz	Fastqsanger.gz
	SRR22020013.fastq.gz	Fastqsanger.gz
	SRR22020014.fastq.gz	Fastqsanger.gz
	SRR22020015.fastq.gz	Fastqsanger.gz
	SRR22020016.fastq.gz	Fastqsanger.gz

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In vivo growth of subclones derived from Lewis lung carcinoma is determined by the tumor microenvironment not intrinsic proliferative ability

Project description:Heterogeneity is a fundamental feature of human tumors and plays a major role in drug resistance and disease progression. In the present study, we selected single-cell-derived cell lines (SCDCLs) derived from Lewis lung carcinoma (LLC1) cells to investigate tumorigenesis and heterogeneity. SCDCLs were generated using limiting dilution. Five SCDCLs were subcutaneously injected into wild-type C57BL/6N mice; however, they displayed significant differences in tumor growth. Subclone SCC1 grew the fastest in vivo, whereas it grew slower in vitro. The growth pattern of SCC2 was the opposite to that of SCC1. Genetic differences in these two subclones showed marked differences in cell adhesion and proliferation. Pathway enrichment results indicate that signal transduction and immune system responses were the most significantly altered functional categories in SCC2 cells compared to SCC1 cells in vitro. The number and activation of CD3+ and CD8+ T cells and NK cells in the tumor tissue of tumor-bearing mice inoculated with SCC2 were significantly higher, whereas those of myeloid cells were significantly lower, than those in the SCC1 and LLC1 groups. Our results suggest that the in vivo growth of two subclones derived from LLC1 was determined by the tumor microenvironment rather than their intrinsic proliferative cell characteristics.

2023-10-23 | GSE216396 | GEO

Expression data from the subclones of Lewis Lung Carcinoma (LLC) cells

Project description:Medium conditioned by LLC cells stimulates thermogenic gene expression when added onto primary adipocytes. We generated single cell colonies from parental LLC cells. Media conditioned by the subclones stimulated thermogenic gene expression in primary adipocytes at varying degrees. Subclones 2, 3, 6 and 28 produce significantly larger amount of thermogenic activity than the subclones 18, 19, 23 and 24. We compared gene expression profiles of these subclones to identify secreted factors enriched in the more thermogenic clones.

2014-07-13 | GSE57797 | GEO

Expression data from the subclones of Lewis Lung Carcinoma (LLC) cells

2014-07-13 | E-GEOD-57797 | biostudies-arrayexpress

Melanerpes lewis

Project description:Melanerpes lewis

| PRJNA960563 | ENA

In vivo growth of subclones derived from Lewis lung carcinoma is determined by the tumor microenvironment.

Project description:Heterogeneity is a fundamental feature of human tumors and plays a major role in drug resistance and disease progression. In the present study, we selected single-cell-derived cell lines (SCDCLs) derived from Lewis lung carcinoma (LLC1) cells to investigate tumorigenesis and heterogeneity. SCDCLs were generated using limiting dilution. Five SCDCLs were subcutaneously injected into wild-type C57BL/6N mice; however, they displayed significant differences in tumor growth. Subclone SCC1 grew the fastest in vivo, whereas it grew slower in vitro. The growth pattern of SCC2 was the opposite to that of SCC1. Genetic differences in these two subclones showed marked differences in cell adhesion and proliferation. Pathway enrichment results indicate that signal transduction and immune system responses were the most significantly altered functional categories in SCC2 cells compared to those in SCC1 cells in vitro. The number and activation of CD3+ and CD8+ T cells and NK cells in the tumor tissue of tumor-bearing mice inoculated with SCC2 were significantly higher, whereas those of myeloid cells were significantly lower, than those in the SCC1 and LLC1 groups. Our results suggest that the in vivo growth of two subclones derived from LLC1 was determined by the tumor microenvironment rather than their intrinsic proliferative cell characteristics.

| S-EPMC9729899 | biostudies-literature

Lewis Lung Carcinoma UROD

Project description:Lewis Lung Carcinoma UROD

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Transcriptomic profile of Sialyl-Lewis X (CD15s)+ basophils and Sialyl Lewis X- basophils

Project description:Sialyl Lewis X (sLexX or CD15s) is a tetra-saccharide N-acetylneuraminic acid, galactose, fucose and N-acetylglucosamine (NeuAcα2,3Galβ1,4(Fucα1,3)-GlcNAc). CD15s is present on various leukocytes usually as O-linked glycans bound to surface molecules such as PSGL-1, CD43 and CD44. On leukocytes, CD15s plays crucial role in extravasation through the interaction with E-selection expressed ion surface of the activated epithelial blood vessels. We find in approximately 10% of individuals basophils lack CD15s on their surface and these basophils fail to roll on E-selectin coated surfaces. In humans, six α(1,3)-fucosyltransferases (FT), α(1,3)-FTs: FT3/FT4/FT5/FT6/FT7/FT9) catalyses the transfer of fucose to the terminal lactosaminyl glycans giving rise to Lewis X (CD15) and/or sialyl Lewis X (CD15s). To identify which enzyme(s), we perform RNA-sequencing of CD15shigh(CD15s Pos) and CD15slow (CD15s Neg) basophils from 6 individuals.

2021-06-15 | GSE122281 | GEO

Prospective Isolation and Characterization of Genetically and Functionally Distinct AML Subclones

Project description:Intra-tumour heterogeneity caused by clonal evolution is a major problem in cancer treatment stratification. To address this problem, we performed label-free quantitative proteomics on primary acute myeloid leukemia (AML) samples. We identified 50 leukemia-enriched plasma membrane (PM) proteins enabling the prospective isolation of genetically distinct subclones from individual AML patients. Subclones differed in their regulatory phenotype, drug sensitivity, growth and engraftment behavior, as determined by RNA sequencing, DNaseI hypersensitive site mapping, transcription factor occupancy analysis, in vitro culture and xenograft transplantation. Finally, we show that these markers can be used to identify and longitudinally track distinct leukemic clones in patients in routine diagnostics. Our study describes a strategy for a major improvement in stratifying cancer diagnosis and treatment.

2018-07-26 | GSE117667 | GEO

human HG-3 B-lymphome cell line subclones

Project description:Genetic heterogeneity though common in tumors has been rarely documented in cell lines. To examine how often B-lymphoma cell lines are comprised of subclones, we performed immunoglobulin (Ig) heavy chain hypermutation analysis. Revealing that subclones are not rare in B-cell lymphoma cell lines, 6/49 Ig hypermutated cell lines (12%) consisted of subclones with individual Ig mutations. Subclones were also identified in 2/284 leukemia/lymphoma cell lines exhibiting bimodal CD marker expression. We successfully isolated 10 subclones from four cell lines HG3, SU-DHL-5, TMD-8, U-2932). Whole exome sequencing was performed to molecularly characterize these subclones. We in detail describe the clonal structure of cell line HG3, derived from chronic lymphocytic leukemia. HG3 consists of three lineages each bearing clone-specific aberrations, gene expression and DNA methylation patterns. While donor patient leukemic cells were CD5+ , two of three HG3 subclones had independently lost this marker. CD5 on HG3 cells was regulated by epigenetic/transcriptional mechanisms rather than by alternative splicing as reported hitherto. In conclusion, we show that the presence of subclones in cell lines carrying individual mutations and characterized by sets of differentially expressed genes is not uncommon. We show also that these subclones can be useful isogenic models for regulatory and functional studies.

2016-09-30 | E-MTAB-4955 | biostudies-arrayexpress

Rattus norvegicus strain:Lewis

Project description:Rattus norvegicus Lewis Targeted Locus (Loci)

| PRJNA171876 | ENA