Project description:To identify molecular characteristics of mouse AMs stimulated with or without Poly(I:C) in the presence of vehicle or MSDC overnight in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of cytokine gene profiles were reduced following MSDC treatment in WT AMs, suggesting that MSDC treatment recuced inflammatory response in AMs.

2023-12-02 | GSE216564 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes following DON treatment

Project description:To identify molecular characteristics of WT AM with or without DON stimulation in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following DON treatment in WT AMs, suggesting that DON treatment altered the balance of proliferative versus inflammatory genes in AMs, and glutamine metabolism is important in regulating AM self-renewal ability.

2023-12-31 | GSE210076 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes [bulk RNA-seq]

Project description:To identify molecular characteristics of WT AM without Wnt3a, and WT, b-catenin-deficient or HIF-1a-deficient AM with Wnt3a stimulation in vitro, we isolated RNA from AMs with various treatments and examined by bulk RNA-seq. We found a large number of gene profiles were altered following Wnt3a treatment in WT AMs, while b-catenin and HIF-1a deficiency largely abolished the effects of Wnt3a treatment on AM transcription, suggesting that b-catenin and HIF-1a mediates most downstream effects of Wnt3a in AMs. Indeed,HIF-1a-deficient AMs essentially phenocopied those of b-catenin-deficient AMs, with more than 80% of differentially expressed genes in HIF-1a-deficient AMs overlapping with b-catenin-deficient AMs following Wnt3a treatment.

2021-05-05 | GSE164791 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) heterogeneity following Wnt3a treatment [single-cell RNA-seq]

Project description:Characterization of in vitro cultured alveolar macrophages (AM) heterogeneity following Wnt3a treatment [single-cell RNA-seq]

| PRJNA691959 | ENA

Transcriptomes of Alveolar epithelial cells

Project description:Freshly isolated type I and type II alveolar epithelial cells were investigated for transcriptomes with dual-color DNA microarray using 3DNA 50 kit.

2010-06-11 | E-GEOD-2160 | biostudies-arrayexpress

Transcriptome analysis of cultured human alveolar epithelial type 2 cells

Project description:We investigated whether in vitro expansion of human alveolar epithelial type II cells is possible. We found that human endogenous human alveolar epithelial type II cells can be cultured and passaged. The culture system enabled retroviral gene transduction into human alveolar epithelial type II cells. We performed RNA sequencing of human alveolar epithelial type II cells transduced with mutant surfactant protein C or control vector.

2019-06-27 | GSE128291 | GEO

Characterization of in vitro cultured alveolar macrophages (AM) transcriptomes and heterogeneity following Wnt3a treatment

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-05-05 | GSE164793 | GEO