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The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

ABSTRACT: The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

PROVIDER: PRJNA894706 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22059372_1.fastq.gz	Fastqsanger.gz
	SRR22059372_2.fastq.gz	Fastqsanger.gz
	SRR22059373_1.fastq.gz	Fastqsanger.gz
	SRR22059373_2.fastq.gz	Fastqsanger.gz
	SRR22059374_1.fastq.gz	Fastqsanger.gz

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The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

Project description:The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

| PRJNA894707 | ENA

The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

Project description:We executed CUT&RUN-seq for SOX2 a different mouse strain E14TG2a

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The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

Project description:We executed ChIPseq for SOX2 a different mouse strain E14TG2a.

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The study of histone modifications and SOX2 occupancy in asynchronous and mitotic mESCs.

Project description:We performed ChIP-seq for H3K4me3, H3K27ac, H3K4me1, H3K27me3, H3K36me3, H3K9me3, and H4K20me3 to characterize chromatin states and investigated SOX2 deposition in asynchronous and mitotic cells.

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The study of SWI/SNF subunits and other transcription factors deposition in asynchronous and mitotic mESCs and cells released from mitosis

Project description:We executed CUT&RUN-seq for SWI/SNF components ARID1A, BRD9, SMARCA4, SMARCB1, SMARCE1, as well as ESRRB, SOX2, and EZH2 in asynchronous and mitotic cells and reported that, in asynchronous cells, ARID1A localized primarily at enhancer regions and EZH2 preferentially deposited at bivalent promoters and silent enhancer domains. The remaining factors were enriched at both TSS/promoters and to varying degrees at active enhancers. Unexpectedly, in mitosis, the chromatin regulatory factors almost all tethered at proximal gene regions with very little binding at enhancers. While the SWI/SNF subunits were bound principally at promoters, EZH2, the catalytic subunit of Polycomb Repressive Complex 2 was bound at both promoters and silent enhancers in mitotic cells. Moreover, we reported that upon the degradation of SMARCE1 in mitosis, the occupancy of SOX2, ESRRB, and EZH2 on mitotic chromatin was disrupted.

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SERBP1 iCLIP in mitotic and asynchronous cells with Blu577 treatment

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2021-10-05 | E-MTAB-10830 | biostudies-arrayexpress

Mus musculus isolate:ES-E14TG2a

Project description:Mus musculus isolate:ES-E14TG2a Raw sequence reads

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