Project description:Comparison and functional analysis of odorant-binding proteins and chemosensory proteins in two closely related thrips species, Frankliniella occidentalis and Frankliniella intonsa (Thysanoptera: Thripidae)
Project description:Frankliniella occidentalis is an important insect carrier of virus disease that harms cash crops, and it often causes virus disease to spread widely in cash crops. Post-translational modifications of proteins play an important role in regulating life activities, and the study of newly discovered modifications (PTMs) is of great significance. Lysine 2-hydroxyisobutyryl (Khib) is an evolutionary conservative form of post-translational modification of a new type of protein discovered in mammals, plants, yeast and pathogenic bacteria in recent years. The study of this modification can increase our understanding of Frankliniella occidentalis, help further study the biological function mechanism of Frankliniella occidentalis and the key to disease transmission, and is of great significance to disease prevention and control.
Project description:Frankliniella occidentalis is an important insect carrier of virus disease that harms cash crops, and it often causes virus disease to spread widely in cash crops. Post-translational modifications of proteins play an important role in regulating life activities, and the study of newly discovered modifications (PTMs) is of great significance. Lysine Klac (Kla) is an evolutionary conservative form of post-translational modification of a new type of protein discovered in mammals, plants, yeast and pathogenic bacteria in recent years. The study of this modification can increase our understanding of Frankliniella occidentalis, help further study the biological function mechanism of Frankliniella occidentalis and the key to disease transmission, and is of great significance to disease prevention and control.
2022-07-19 | PXD030799 | Pride
Project description:Frankliniella occidentalis transcriptome analysis
Project description:Paleoproteomics is the proteomics analysis of ancient proteins, which may be better preserved than DNA in fossil inclusions and particularly in amber fossilized tree resin. However, only yeast proteins have been previously suggested as identified by paleoproteomics analysis conducted in fossil amber. In this study, we developed and applied a paleoproteomics approach to study fossil arthropod inclusions in five Burmese (also known as Burmite and Kachin) amber (Cretaceous, ca. 99 mya) pieces. The results of morphological analysis supported the first report of fossil scavenger mite (Holothyrida, Neothyridae) together with co-inclusions of putative lacewing larva (Neuroptera, Chrysopidae) and thrip Frankliniella sp. (Thysanoptera, Thripidae), the identification of Cornupalpatum sp. (Ixodidae), and two mites Prostigmata (Acari, Trombidiformes, Tetranychidae). A control amber fragment derived from the piece with mite-lacewing-thrip co-inclusions was included in the analysis. The paleoproteomics analysis was conducted by Mass Spectrometry (MS) and Liquid Chromatography-Tandem Mass Spectrometry by searching results from all fossil amber pieces against a compile database containing all sequences from Acari, Insecta and Enterobacterales taxonomies in Uniprot together with contaminants database supplemented with human keratins and bovine trypsin. Identified proteins were selected for analysis based on those identified by MS with more than two peptides per protein and at least one peptide with 1% FDR. Paleoproteomics identified protein orthologs present in fossil amber inclusions of ca. 99 mya in arthropods and other organisms. These results provide a paleoproteomics approach to complement morphological studies in amber inclusions.
2026-03-23 | PXD066794 | Pride
Project description:The complete mitogenome of Odontothrips phaseoli (Thysanoptera: Thripidae) and phylogenetic analysis
| PRJNA1021028 | ENA
Project description:Frankliniella occidentalis female transcriptome
| PRJNA833754 | ENA
Project description:Frankliniella occidentalis dehydration transcriptome