Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells

ABSTRACT: Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells

PROVIDER: PRJNA896621 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22133496.fastq.gz	Fastqsanger.gz
	SRR22133497.fastq.gz	Fastqsanger.gz
	SRR22133498.fastq.gz	Fastqsanger.gz
	SRR22133499.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells

Project description:Neural stem/progenitor cells (NSPCs) exhibited a selective neuron and oligodendrocyte differentiation on the novel conductive, flexible biopolymer composite (PUCL@CNT).

2023-05-10 | GSE217049 | GEO

archaea-cnt

Project description:archaea-cnt Raw sequence reads

| PRJNA478110 | ENA

Salinispora mooreana strain:CNT-150

Project description:Salinispora pacifica CNT-150 transcriptomics - CNT-150-216H-1

| PRJNA415665 | ENA

Salinispora mooreana strain:CNT-150

Project description:Salinispora pacifica CNT-150 transcriptomics - CNT-150-216H-3

| PRJNA415648 | ENA

Salinispora mooreana strain:CNT-150

Project description:Salinispora pacifica CNT-150 transcriptomics - CNT-150-96H-3

| PRJNA415664 | ENA

Salinispora mooreana strain:CNT-150

Project description:Salinispora pacifica CNT-150 transcriptomics - CNT-150-96H-1

| PRJNA415663 | ENA

bacteria for CNT

Project description:bacteria for CNT Raw sequence reads

| PRJNA478106 | ENA

Mechano-Hypoxia Conditioning of Engineered Human Meniscus

Project description:Meniscus fibrochondrocytes (MFCs) experience simultaneous hypoxia and mechanical loading in the knee, conditions that have promising applications in human meniscus tissue engineering. We hypothesized that “mechano-hypoxia conditioning,” using mechanical loading such as dynamic compression (DC) and cyclic hydrostatic pressure (CHP), would enhance development of human meniscus fibrocartilage extracellular matrix in vitro. MFCs from inner human meniscus surgical discards were pre-cultured on porous type I collagen scaffolds with TGF-β3 supplementation to form baseline tissues with newly-formed matrix. They were then treated with DC or CHP under hypoxia (HYP, 3% O2) for 5 days. DC was the more effective load regime, and combined HYP/DC enhanced gene expression of fibrocartilage precursors. The individual treatments of DC and HYP regulated thousands of genes and combined in an overwhelmingly additive rather than synergistic manner. Baseline tissues were then treated with a short course of DC (5 vs 60 minutes, 10-20% vs 30-40% strain) with different pre-culture durations (3 vs 6 weeks). Longer courses of loading had diminishing returns in terms of gene regulation. There was a dose-effect for higher DC strains, whereas outcomes were mixed for different MFC donors in pre-culture durations. Finally, baseline tissues were conditioned for 3 weeks with mechano-hypoxia conditioning to assess mechanical and protein-level outcomes. There were 1.8 to 5.1-fold gains in the dynamic modulus relative to baseline in HYP/DC, but matrix outcomes were equal or inferior to static controls. Long-term mechano-hypoxia conditioning was effective in suppressing hypertrophic markers (e.g., COL10A1 10-fold suppression vs static/normoxia). Applied appropriately, mechano-hypoxia conditioning can support meniscus fibrocartilage development in vitro and may be useful as a strategy for developing non-hypertrophic articular cartilage using mesenchymal stem cells.

2021-08-23 | GSE180467 | GEO

Salinispora arenicola CNT-088

Project description:Salinispora arenicola CNT-088 Genome sequencing and assembly

| PRJNA84269 | ENA

Nocardiopsis oceanisediminis strain:CNT-189

Project description:Nocardiopsis oceanisediminis strain:CNT-189 Genome sequencing

| PRJNA706861 | ENA