Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: EccDNA of a CHO-K1 clone

PROVIDER: PRJNA896947 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22143436_1.fastq.gz	Fastqsanger.gz
	SRR22143436_2.fastq.gz	Fastqsanger.gz
	SRR22143437_1.fastq.gz	Fastqsanger.gz
	SRR22143437_2.fastq.gz	Fastqsanger.gz
	SRR22143438_subreads.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

CHO cells: Serum free adapted cells (Control) vs. Serum adapted cells

Project description:Transcriptional profiling of CHO-K1 cells comparing to in-house serum-free and suspension adapted CHO-K1 cells in the exponential phase. Goal was to determine the effects of serum on CHO-K1 cells.

2016-12-24 | GSE92885 | GEO

Cricetulus griseus strain:CHO-K1 Clone A11

Project description:Cricetulus griseus strain:CHO-K1 Clone A11 Genome sequencing

| PRJNA579347 | ENA

Transcriptomic analysis of H1299, HEK293T and CHO-K1 treated with Filgotinib

Project description:RNA-sequencing was performed on H1299, HEK293T and CHO-K1 transfected with plasmids expressing EGFP and mKate in an open loop circuit (OL), w/wo compound supplementation, or with iFFL circuit.mKate has target sites for miRNAs highly expressed in the different cell lines (miRNA31 for H1299, miRNA221 for HEK293T and miRNA21 for CHO-K1)

2025-07-24 | E-MTAB-15146 | biostudies-arrayexpress

Expression profile of HER2-overexpressing CHO cells treated with trastuzumab and pertuzumab

Project description:CHO-K1 (wildtype) and CHO-K6 (stablely overexpressing human HER2) cells were terated with 10 ug/ml transtuzumab or pertuzumab and their combination for 24 h and then the whole gene expression was analyzed by cDNA array.

2018-08-29 | GSE110189 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of miR-29b-1 and miR-29a targets in CHO-K1 cells

Project description:The goal of this experiment was to identify the putative mRNA targets of miR-29b-1 and miR-29a in CHO-K1 cells

2019-08-01 | GSE85710 | GEO

Investigating Subpopulation Dynamics in Clonal CHO-K1 Cells with Single-Cell RNA Sequencing

Project description:CHO cells have primarily been studied in bulk, assuming that these bulk samples are identical because of genetic clonality across the sample. In this study, we performed single-cell RNA sequencing on two clonal CHO-K1 cell populations with different stability phenotypes over a 90 day culture period.

2024-09-02 | GSE275930 | GEO

Project description:CHO-K1 transcriptome sequencing

| PRJEB4847 | ENA

A Chinese hamster transcription start site atlas that enables targeted editing of CHO cells

Project description:Gene expression is a key determinant of phenotypes that made Chinese Hamster Ovary (CHO) cells, with their human-like glycosylation profile and high protein titers, one of the most widely used cells for the production of therapeutic proteins and biopharmaceuticals. Engineering CHO gene expression thus holds a key to improve drug quality and cost effective production. However, the success of engineering gene expression or ectopic activation of silent genes to optimize desired pathways requires accurate annotation of the underlying regulatory elements and the transcription start site (TSS). Unfortunately, to date, most TSSs of CHO-expressed genes and the ~50% of hamster genes that are silent in CHO were computationally predicted and are frequently inaccurate. To oust this hurdle, we report revised TSSs annotations for 15,308 Chinese Hamster genes and 4,145 non-coding RNAs based on experimental data from CHO K1 cells and 10 hamster tissues. In the example of the glycosyltransferase gene Mgat3, we further demonstrate how accurate annotations readily facilitate activating silent genes by CRISPRa. Together, we envision that our annotation and data from the Chinese Hamster will provide a rich resource for the CHO community, improve genome engineering efforts and additionally aid comparative and evolutionary studies.

2020-10-06 | GSE159044 | GEO

Revealing suspension adapation of CHO cell in serum free media

Project description:Purpose : We performed strand-specific RNA-seq analysis of CHO cells according to adaptation trajectory to identify the specific genes responded to the suspension adaptation. Result : Cell growth, replication, and nucleotide metabolism categories were enriched in up regulated clusters whereas cellular community category which contains focal adhesion related genes were enriched in down regulated clusters CHO-K1 mRNA profiles during suspension adaptation generated by RNA sequencing, in duplicate, using Illumina MiSeq.

2015-11-19 | E-GEOD-75094 | biostudies-arrayexpress

Cricetulus griseus

Project description:Phenobarbital exposed CHO-K1 cells

| PRJDB5737 | ENA