Project description:To investigate class A G protein-coupled receptors (GPCR)-targeted drugs in the regulation of osteogenic differentiation, we investigated the effects of drugs using mesenchymal stromal cells. By applying microarray dataset, we idntified the mRNA expressions profiles in hDPSCs.

2023-01-20 | GSE217436 | GEO

Targeting class A GPCRs for hard tissue regeneration [ATAC-seq]

2023-01-20 | GSE217264 | GEO

Targeting class A GPCRs for hard tissue regeneration [RNA-seq]

Project description:To investigate class A G protein-coupled receptors (GPCR)-targeted drugs in the regulation of osteogenic differentiation, we investigated the effects of drugs using mesenchymal stromal cells. We conducted whole-transcriptome analysis using bulk RNA sequencing (RNA-seq) of six drugs.

2023-01-20 | GSE217371 | GEO

Targeting class A GPCRs for hard tissue regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-01-20 | GSE217437 | GEO

First-in-class MKK4 inhibitors enhance liver regeneration and prevent liver failure

Project description:<p>Diminished hepatocyte regeneration is a key feature of acute and chronic liver diseases and after extended liver resections, resulting in the inability to maintain or restore a sufficient functional liver mass. Therapies to restore hepatocyte regeneration are lacking, making liver transplantation the only curative option for end-stage liver disease. Here, we report on the structure-based development and characterization (nuclear magnetic resonance [NMR] spectroscopy) of first-in-class small molecule inhibitors of the dual-specificity kinase MKK4 (MKK4i). MKK4i increased liver regeneration upon hepatectomy in murine and porcine models, allowed for survival of pigs in a lethal 85% hepatectomy model, and showed antisteatotic and antifibrotic effects in liver disease mouse models. A first-in-human phase I trial (European Union Drug Regulating Authorities Clinical Trials [EudraCT] 2021-000193-28) with the clinical candidate HRX215 was conducted and revealed excellent safety and pharmacokinetics. Clinical trials to probe HRX215 for prevention/treatment of liver failure after extensive oncological liver resections or after transplantation of small grafts are warranted.</p>

2024-09-23 | MTBLS9434 | MetaboLights

Microarray hybridization data from the root tips of 5-day-old Arabidopsis thaliana seedlings grown on tilted, hard agar surfaces

Project description:The growth behavior of plant roots on tilted, hard agar surfaces is determined by many basic cellular processes, including microtubule dynamics and cell wall expansion. Among Arabidopsis thaliana accessions there is natural variation for these behaviors, including one known as skewing or slanting. The root skewing pattern on hard, tilted agar surfaces may be a clue to adaptations of an accession to its environment. Here, we compare expression profiles of two accessions with diverse skewing behavior grown on the wave assay, which consists of seedlings growing two days vertically and 3 days tilted on hard agar plates. Cvi has a strong skew on tilted, hard agar sufaces, and Ler-2 has a weaker one. We also include a near isogenic line, 170G-55-16 a.k.a HGI2.1, that is mostly Ler-2 in background but has a segment of Cvi introgressed into chromosome 2. This line has an intermediate skew between its two parents. 3 biological replicates of each of 3 genotypes (Cvi, Ler-2, and 170G-55-16/HGI2.1) were subjected to the wave assay. After the assay, approximately 600 root tips from each biological replicate were pooled for RNA extraction and hybridization on the Affymetrix ATH1 microarray.

2011-03-31 | E-GEOD-28275 | biostudies-arrayexpress

Microarray hybridization data from the root tips of 5-day-old Arabidopsis thaliana seedlings grown on tilted, hard agar surfaces

2011-03-31 | GSE28275 | GEO