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Puccinia

ABSTRACT: Puccinia sp. overview

PROVIDER: PRJNA899646 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22247103_1.fastq.gz	Fastqsanger.gz
	SRR22247104_1.fastq.gz	Fastqsanger.gz
	SRR22247105_1.fastq.gz	Fastqsanger.gz
	SRR22247106_1.fastq.gz	Fastqsanger.gz
	SRR22247107_1.fastq.gz	Fastqsanger.gz

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Gene expression analysis of four conditions for Puccinia graminis f. sp. tritici

Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici

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Transcription analysis by RNAseq of adult wheat after inoculation with Puccinia graminis f. sp. tritici

Project description:The RNA sequencing analysis was undertaken to investigate the transcriptomic changes in adult wheat inoculated with Puccinia graminis f. sp. tritici the causal agent of stem rust disease. The project firstly aims to compare gene expression in one susceptible wheat line with two wheat lines exhibiting adult plant resistance to the stem rust. Secondly, the project aims to examine the temporal changes in gene expression in wheat after inoculation. Wheat plants was grown until maturity under greenhouse conditions. Plants were inoculated with Puccinia graminis f. sp. tritici and the flag leaf sheath sampled for RNA sequencing. The project aims to give essential insight into the adult plant resistance response in wheat to Puccinia graminis f. sp. tritici inoculation.

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Gene expression analysis of four conditions for Puccinia graminis f. sp. tritici

2010-12-31 | E-GEOD-25020 | biostudies-arrayexpress

Inoculation of rice with the non-host pathogen Puccinia triticina f. sp. tritici elicits an active transcriptional response

Project description:Japonica rice cultivar Nipponbare was inoculated with wheat leaf rust (Puccinia triticina f. sp. tritici, non-host pathogen to rice) to compare gene expression profiles with mock-inoculated controls. Although eventually failed in invasion, leaf rust induced a set of rice genes that were distinctally up-regulated, some of those were confirmed by quantitative real-time PCR assays.

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Puccinia horiana

Project description:Puccinia horiana overview

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Puccinia silphii

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Transcription profiling time series of leaves from Hordeum vulgare Ingrid infected with Puccinia hordei and Puccinia triticana

Project description:Time course: Interaction of Puccinia hordei with Hordeum vulgare, Ingrid (leaf) and Puccinia triticana with Hordeum vulgare, Ingrid (leaf)

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Transcriptome-wide Association study identifies putative Elicitors/Suppressor of Puccinia graminis f. sp. tritici that Modulate Barley rpg4-mediated Stem Rust Resistance

Project description:Transcriptomic profiling was done on barley samples incoulated with different Puccinia graminis f. sp. tritici (Pgt) isolates of varying virulence profile on barley stem rust resistance gene rpg4/5 and Rpg1. Several differentally expressed host and pathogen genes were identified while comparing gene expression profiles between group of samples inoculated with isoaltes of varying virulence. Several variants with genes of pathogen were idnetified that are possibly associated with virulence to rpg4/5.

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Transcription profiling by array of wheat after inoculation with Puccinia triticina, Puccinia hordei or mock-inoculation to identify candidate genes for nonhost resistance against rust disease

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