Project description:For decades, researchers have devoted great efforts to study the tumor suppressor gene Trp53, however, our understanding of Trp53 regulation has never reached either organism-scale or single-cell resolution. We constructed single-cell transcriptomic landscape covering 18 organs of Trp53-/- mice models to explore genetic network transformations in the absence of Trp53.
Project description:For decades, researchers have devoted great efforts to study the tumor suppressor gene Trp53, however, our understanding of Trp53 regulation has never reached either organism-scale or single-cell resolution. We constructed single-cell epigenomic landscape covering 17 organs of Trp53-/- mice to explore genetic network transformations in the absence of Trp53.
Project description:For decades, researchers have devoted great efforts to studying the tumor suppressor gene Trp53, however, our understanding of Trp53 regulation has never reached either organism-scale or single-cell resolution. Here, we performed spatial RNA analysis to confirm the Trp53 KO-induced tumor initiation states.
Project description:For decades, researchers have devoted great efforts to study the tumor suppressor gene Trp53. However, tissue-specific Trp53 regulation still remains elusive.Here, we used Cut&tag to analyze the p53 target gene groups in 18 tissues and organs of adult mice to decipher the tissue heterogeneity of Trp53 regulation.
Project description:An overview of the expression pattern of all rice genes under natural field conditions based on microarray analysis of different organs and tissues at various stages of growth and development from transplanting to harvesting. A total of 48 samples representing organs/tissues at various stages of growth and development with 3 replicates each except for one anther sample with 2 replicates. Vegetative organs such as leaf blade, leaf sheath, root and stem were sampled during the vegetative, reproductive and ripening stages. Young inflorescence, anther, pistil, lemma, palea, ovary, embryo, and endosperm were sampled at various stages of development.
Project description:To identificate long noncoding RNAs in rice, we profiled transcriptome of various organs at different developmental stages using nondirectional paired-end RNA-seq based on poly(A) selection.