The RHW1-ZCN4 regulatory pathway confers natural variation of husk leaf width in maize
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ABSTRACT:
PROVIDER: PRJNA901414 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR22273800_1.fastq.gz | Fastqsanger.gz | |||
| SRR22273800_2.fastq.gz | Fastqsanger.gz | |||
| SRR22273801_1.fastq.gz | Fastqsanger.gz | |||
| SRR22273801_2.fastq.gz | Fastqsanger.gz | |||
| SRR22273802_1.fastq.gz | Fastqsanger.gz |
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Project description:Maize husk leaf - the outer leafy layers covering the ear - modulates kernel yield and quality. Despite its importance, however, the genetic controls underlying husk leaf development remain elusive. Our previous genome-wide association study identified a single nucleotide polymorphism located in the gene RHW1 (Regulator of Husk leaf Width) that is significantly associated with husk leaf-width diversity in maize. Here, we further demonstrate that a polymorphic 18-bp InDel (insertion/deletion) variant in the 3' untranslated region of RHW1 alters its protein abundance and accounts for husk leaf width variation. RHW1 encodes a putative MYB-like transcriptional repressor. Disruption of RHW1 altered cell proliferation and resulted in a narrower husk leaf, whereas RHW1 overexpression yielded a wider husk leaf. RHW1 positively regulated the expression of ZCN4, a well-known TFL1-like protein involved in maize ear development. Dysfunction of ZCN4 reduced husk leaf width even in the context of RHW1 overexpression. The InDel variant in RHW1 is subject to selection and is associated with maize husk leaf adaption from tropical to temperate regions. Overall, our results identify that RHW1-ZCN4 regulates a pathway conferring husk leaf width variation at a very early stage of husk leaf development in maize.
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Project description:In the present study, we found a new walnut germplasm from wild Juglans cathayensis population, which presented white husk that did not brown. We compared the transcriptome between the fresh-cut browning (control) and white husks of the Chinese walnut using Illumina HiSeq 4000 platform
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Project description:Canopy architecture in cereals plays an important role in determining yield. The width of leaves represents one key aspect of this canopy architecture. However, our understanding of leaf width control in cereals remains incomplete. Classical mutagenesis studies in barely identified multiple morphological mutants, including those with differing leaf widths. Of these, we characterized the broad leaf13 (blf13) mutant in detail. Mutant plants form wider leaves due to increased post-initiation growth and cell proliferation. The mutant phenotype perfectly co-segregated with a semi-dominant, missense mutation in the HvHNT1 gene which affected a highly conserved region of the encoded protein, orthologous to the rice NARROW LEAF1 (NAL1) protein. Causality of this mutation for the blf13 phenotype is further supported by correlative transcriptomic analyses and protein-protein interaction studies showing that the mutant HvNHT1 protein interacts more strongly with a known interactor than wild-type HvHNT1. The mutant HvHNT1 protein also showed stronger homodimerization compared to wild type HvHNT1homodimerization, with a dimer between mutant and wildtype HvHNT1intermediate. Thus, the blf13 mutation parallels known gain-of-function NAL1 alleles in rice that increase leaf width and grain yield, suggesting the blf13 mutation may have a similar agronomic potential in barley.
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