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Exploring the Role of DNMT1 in RNA m⁵C Methylation (RNA-BS-SEQ DNMT1 knockdown)

ABSTRACT: Exploring the Role of DNMT1 in RNA m⁵C Methylation (RNA-BS-SEQ DNMT1 knockdown)

PROVIDER: PRJNA901995 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22302687_1.fastq.gz	Fastqsanger.gz
	SRR22302687_2.fastq.gz	Fastqsanger.gz
	SRR22302688_1.fastq.gz	Fastqsanger.gz
	SRR22302688_2.fastq.gz	Fastqsanger.gz
	SRR22302689_1.fastq.gz	Fastqsanger.gz

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Exploring the Role of DNMT1 in RNA m⁵C Methylation (RNA-BS-SEQ DNMT1 knockdown)

Project description:DNA methyltransferase 1 (DNMT1) is an enzyme known for DNA methylation maintenance. However, point mutations in its RFTS domain lead to late-onset neurodegeneration such as the autosomal dominant cerebellar ataxia-deafness and narcolepsy (ADCA-DN) disorder. Here we demonstrated that wild-type DNMT1 also has the capability to bind to mRNA transcripts and facilitate 5-methylcytosine (m5C) RNA methylation by recruiting NOP2/Sun RNA methyltransferase 2 (NSUN2). RNA m5C methylation, in turn, promotes RNA stability for those genes modulating mitochondrial function. When DNMT1 RFTS domain is mutated in the case of ADCA-DN disorder, it triggers aberrant DNMT1-RNA interaction and significantly elevated m5C RNA methylation and RNA stability for a portion of metabolic genes. Consequently, increased levels of metabolic RNA transcripts contribute to cumulative oxidative stress, mitochondrial dysfunction, and neurological symptoms. Collectively, our results highlight a novel role for DNMT1 in regulating both DNA and RNA methylation as well as mitochondrial function, shedding light on the pathogenic mechanism of DNMT1 mutation-induced neurodegeneration.

2025-05-06 | GSE218053 | GEO

Exploring the Role of DNMT1 in RNA m⁵C Methylation (RNA-BS-SEQ NSUN2 Knock down)

Project description:Exploring the Role of DNMT1 in RNA m⁵C Methylation (RNA-BS-SEQ NSUN2 Knock down)

| PRJNA1228128 | ENA