Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: A Genetically Encoded, Bioluminescent Reporter of Pattern Triggered Immunity in Nicotiana benthamiana

PROVIDER: PRJNA902803 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR22325478.fastq.gz	Fastqsanger.gz
	SRR22325479.fastq.gz	Fastqsanger.gz
	SRR22325480.fastq.gz	Fastqsanger.gz
	SRR22325481.fastq.gz	Fastqsanger.gz
	SRR22325482.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Co-immuno precipitation of the aphid saliva protein Mp10 and the plant de-ubiquitinating enzyme AMSH2

Project description:This dataset corresponds to experiments described in the manuscript “The conserved aphid saliva chemosensory protein effector Mp10 targets plant AMSH deubiquitinases at cellular membranes to suppress pattern-triggered immunity”, and includes mass spectrometry analysis of Co-immuno-precipitation experiments of membrane-enriched fractions of Nicotiana benthamiana leaves transiently co-expression the aphid saliva effector protein Mp10 and it’s interacting plant target AMSH2.

2024-11-06 | PXD055941 | Pride

Co-immuno precipitation of the aphid saliva protein Mp10 and the plant de-ubiquitinating enzyme AMSH2

2024-11-06 | PXD057543 | Pride

Infiltration-RNAseq reveals enhanced defense responses in Nicothiana benthamiana leaves overexpressing the banana MaWRKY45 gene

Project description:Members of the WRKY transcription factor (TF) family play a crucial role in regulating plant defense responses to biotic stress. Here, we report that the constitutive overexpression of the banana MaWRKY45 gene in the leaves of the model plant Nicotiana benthamiana enhances the transcript levels of immune response genes. Among the genes that were up-regulated, we identified those involved in PAMP-triggered immunity, effector-trigger immunity and systemic acquired resistance, among others. These results provide insights into the molecular mechanisms triggered downstream of a single TF such as the banana MaWRKY45. Our findings suggest that MaWRKY45 is a promising candidate for banana breeding programs focused on enhancing disease resistance.

2025-02-12 | GSE282538 | GEO

Project description:Genetically encoded fluorescent reporter for polyamines

| PRJNA1185204 | ENA

Ectopic expression of URT1 remodels poly(A) tail profiles in Nicotiana benthamiana

Project description:3’RACE-seq experiments were conducted to determine the impact of URT1 ectopic expression on the poly(A) tail length profile of both a GFP reporter mRNA co-expressed in Nicotiana benthamiana leaves and an endogenous mRNA encoding PATHOGENESIS-RELATED PROTEIN 2 (PR2)

2020-05-28 | GSE148409 | GEO

NGS data of Nicotiana benthamiana 16c plants after CRISPR/Cas9 gene editing

Project description:Provided data came from a detailed study on Nicotiana benthamiana 16c plants where we use Tobacco Rattle Virus (TRV) as a molecular switch to change the chromatin state of a reporter gene (P35S::GFP) from an actively transcribed to a transcriptionally silenced state. Our approach enables us to interrogate different chromatin states of the same locus with the same set of CRISPR/Cas9 genome editing reagents and systematically describe the effect of chromatin state on the frequency and type of mutations induced at various Cas9 targets in a huge set of independently edited cells.

2022-05-04 | E-MTAB-10256 | biostudies-arrayexpress

Discovery of novel proteins in Agrobacterium tumefaciens to improve molecular pharming yields of Trastuzumab

Project description:PlantForm Inc. (https://www.plantformcorp.com) is a Canadian biopharmaceutical company that produces antibody drugs using molecular pharming, where a genetically modified strain of Agrobacterium tumefaciens (strain At564A, derived from C58) transforms a genetically modified strain of Nicotiana benthamiana KDFX plant line (derived from USDA accession “TW16”) for biopharmaceutical expression. However, further research is required to comprehensively profile the proteome and secretome of At564A in their pipeline to identify areas of improvement. Using mass spectrometry-based bottom-up proteomics, we identified significantly altered abundance of virulence proteins after inducing virulence using acetosyringone and plant leaf extract from Nicotiana benthamiana.

2024-10-13 | PXD047349 | Pride

The transcriptional landscape of Nicotiana benthamiana and Verticillium dahliae interaction

Project description:Verticillium dahliae is a soil-borne fungus with a broad host range, including the model plants Nicotiana benthamiana and Arabidopsis thaliana. The plant immunity can be activated by Vd-derived patterns while V. dahliae secreted effectors. We report here the RNAseq analyses of samples from N. benthamiana infected by V. dahliae strains 592 and 171 at different time points. The data showed dynamic expression patterns of genes not only from plant defense signaling but also plant developmental signaling.

2023-07-13 | GSE236729 | GEO

Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1

Project description:Pattern-triggered immunity (PTI) is a central component of plant immunity. Activation of PTI relies on the recognition of microbe-derived structures, termed patterns, through plant encoded surface-resident pattern recognition receptors (PRRs). We have identified proteobacterial translation initiation factor 1 (IF1) as an immunogenic pattern that triggers PTI in Arabidopsis thaliana and some related Brassicaceae species.

2022-03-03 | PXD031124 | Pride

Brachypodium distachyon strain:Bd21

Project description:Pattern-triggered immunity in Brachypodium distachyon

| PRJDB13671 | ENA