Project description:This study investigates the metabolic responses of two Zoysia species (Z. macrostachya and Z. sinica) adapted to distinct microhabitats in the Yellow River Delta. Leaf samples were collected from natural populations growing under different saline-alkaline conditions. Untargeted metabolomics was performed using LC-MS in both positive and negative ion modes to profile the metabolite composition. The results revealed significant differences in key metabolic pathways, including tryptophan metabolism, flavonoid biosynthesis, and phenylpropanoid metabolism, between the two species, providing insights into their differential adaptation to saline-alkaline stress.
Project description:The fate of the carbon stocked in permafrost soils following global warming and permafrost thaw is of major concern in view of the potential for increased CH4 and CO2 emissions from these soils. Complex carbon compound degradation and greenhouse gas emissions are due to soil microbial communities, but their composition and functional potential in permafrost soils are largely unknown. Here, a 2 m deep permafrost and its overlying active layer soil were subjected to metagenome sequencing, quantitative PCR, and microarray analyses. The active layer soil and 2 m permafrost soil microbial community structures were very similar, with Actinobacteria being the dominant phylum. The two soils also possessed a highly similar spectrum of functional genes, especially when compared to other already published metagenomes. Key genes related to methane generation, methane oxidation and organic matter degradation were highly diverse for both soils in the metagenomic libraries and some (e.g. pmoA) showed relatively high abundance in qPCR assays. Genes related to nitrogen fixation and ammonia oxidation, which could have important roles following climatic change in these nitrogen-limited environments, showed low diversity but high abundance. The 2 m permafrost soil showed lower abundance and diversity for all the assessed genes and taxa. Experimental biases were also evaluated and showed that the whole community genome amplification technique used caused large representational biases in the metagenomic libraries. This study described for the first time the detailed functional potential of permafrost-affected soils and detected several genes and microorganisms that could have crucial importance following permafrost thaw.
2010-03-08 | GSE20073 | GEO
Project description:Soil fungi in Yellow River delta
| PRJNA1235502 | ENA
Project description:Yellow River Delta coastal bacterial community
| PRJNA532461 | ENA
Project description:Microorganisms in the Yellow River Delta