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Bulk RNA-seq expression data isolated from early L1 stage C. elegans larvae

ABSTRACT: Bulk RNA-seq expression data isolated from early L1 stage C. elegans larvae

PROVIDER: PRJNA903191 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22338940.fastq.gz	Fastqsanger.gz
	SRR22338941.fastq.gz	Fastqsanger.gz
	SRR22338942.fastq.gz	Fastqsanger.gz
	SRR22338943.fastq.gz	Fastqsanger.gz

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Bulk RNA-seq expression data isolated from early L1 stage C. elegans larvae

Project description:During development C. elegans GABAergic dorsal D-class (DD) motor neurons reverse polarity such that the axonal and dendritic compartments are switched. We found that the gene DVE-1 is important for this process. To elucidate potential targets of dve-1 transcriptional regulation we conducted bulk RNA sequencing of total RNA from early L1 stage wild type and dve-1 mutant animals

2024-12-08 | GSE218349 | GEO

Bulk RNA-seq expression data isolated from early L1 and L4 stage C. elegans larvae

Project description:Bulk RNA-seq expression data isolated from early L1 and L4 stage C. elegans larvae

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Expression data from DD neurons isolated from early L1 stage C. elegans larvae.

Project description:Six DD class GABAergic neurons are generated in the embryo to synapse with ventral muscles and receive input from cholinergic neurons in the dorsal nerve cord. After hatching and toward the end of the first larval (L1) stage, DD neurons reverse polarity (i.e., synapse with dorsal muscles, receive ventral cholinergic inputs). Expression profiles were generated from DD neurons in the early L1 stage before the initiation of the remodeling program. We used microarray analysis to detect transcripts with potential roles in DD remodeling. We used FACS to isolate ttr-39::mCherry labeled DD GABAergic motor neurons from a synchronized population of L1 larvae and amplified and labeled total RNA to generate Affymetrix Genome Array data. The DD data sets were compared to an expression profile obtained from all cells in a matched population of synchronized L1 larvae. See Spencer et al. PLOS One 9, e112102 (2014).

2015-08-01 | E-GEOD-71618 | biostudies-arrayexpress

Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

Project description:Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

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Bulk RNA-seq expression data isolated from early L4 stage C. elegans larvae

2024-12-08 | GSE218350 | GEO

Expression data from DD neurons isolated from early L1 stage C. elegans larvae.

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mRNA-seq comparison of L1 larvae experiencing osmotic and starvation-induced arrest

Project description:Wild-type (N2) C. elegans embryos were plated with or without E. coli OP50 as food and with 100mM or 500mM NaCl at 25C (4 conditions). They were allowed to develop for 24hr and collected as L1 larvae for RNA-seq.

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Expression data from Caenorhabditis elegans L1-stage larvae exposed to ethidium bromide and/or serial ultraviolet C radiation

Project description:Ultraviolet C radiation (UVC) damages the nuclear and mitochondrial genomes; this damage is repaired in the nuclear but not mitochondrial genome. Ethidium bromide (EtBr) inhibits mitochondrial DNA replication. We were interested in the transcriptomic response to exposure to UVC, EtBr, and the combination. The UVC exposure protocol results in a high level of mitochondrial DNA damage, and a low level of nuclear DNA damage (because of repair). We exposed age-matched L1-stage Caenorhabditis elegans to ultraviolet C radiation (UVC ) three times, separated in time by 24 h, in the absence of food. After the third exposure, larvae were placed on K agar plates with OP50 bacterial food. In some cases ethidium bromide was also used. Nematodes were sampled for RNA isolation several times.

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DamID of Dam::EMR-1 in Caenorhabditis elegans L1 larvae

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Wild-type and daf-16(mgDf50) L1 larvae 3 hours after hatching in the presence or absence of food

Project description:This experiment was designed to identify early targets of daf-16/FoxO during L1 starvation in C. elegans. Previous work (Baugh et al 2009) examined temporal dynamics of gene expression in fed and starved L1 larvae using the same platform and methods. Here, a single time point was analyzed (~3 hr after hatching) in fed and starved larvae also comparing wild-type and a daf-16 null mutant. Statistical analysis includes pairwise comparisons between the four conditions as well as a two-factor analysis to explicitly identify genes affected by nutrient availability in daf-16-dependent fashion.

2017-05-24 | GSE99201 | GEO

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