Bioinformatics Reveal the Potential Molecular Mechanism of Agrimol B against Osteosarcoma
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA906017 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR22426850_1.fastq.gz | Fastqsanger.gz | |||
| SRR22426850_2.fastq.gz | Fastqsanger.gz | |||
| SRR22426851_1.fastq.gz | Fastqsanger.gz | |||
| SRR22426851_2.fastq.gz | Fastqsanger.gz | |||
| SRR22426852_1.fastq.gz | Fastqsanger.gz |
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Project description:Despite the advance in diagnosis and treatment, the prognosis of osteosarcoma patients remains unsatisfied. Therefore, it is imperative to identify novel therapeutic targets for osteosarcoma. Through RNA sequencing (RNA-seq) combined with functional screening, N4-acetylcytidine (ac4C) acetyltransferase 10 (NAT10) was identified as a candidate therapeutic target in osteosarcoma. Upregulated NAT10 correlated with poor prognosis in osteosarcoma patients and NAT10 knockout drastically inhibited cell proliferation and metastasis in vitro and in vivo. NAT10 enhanced mRNA stability and translation efficiency of activating transcription factor 4 (ATF4) through ac4C modification. ATF4 induced transcription of asparagine synthetase (ASNS), which catalyzes asparagine (Asn) biosynthesis. Asn promote protein and nucleotide synthesis, facilitating osteosarcoma progression. Overexpression of ATF4, ASNS or supplementation of asparagine rescue the tumor inhibitory effect of NAT10 knockout.
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