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Short-term effects of human versus bovine sialylated milk oligosaccharide microinjection on zebrafish larvae survival, locomotor behavior and gene expression

ABSTRACT: Short-term effects of human versus bovine sialylated milk oligosaccharide microinjection on zebrafish larvae survival, locomotor behavior and gene expression

PROVIDER: PRJNA906024 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22426879_1.fastq.gz	Fastqsanger.gz
	SRR22426879_2.fastq.gz	Fastqsanger.gz
	SRR22426880_1.fastq.gz	Fastqsanger.gz
	SRR22426880_2.fastq.gz	Fastqsanger.gz
	SRR22426881_1.fastq.gz	Fastqsanger.gz

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Short-term effects of human versus bovine sialylated milk oligosaccharide microinjection on zebrafish larvae survival, locomotor behavior and gene expression

Project description:Milk oligosaccharides are a complex class of carbohydrates that act as bioactive factors in numerous defensive and physiological functions, including brain development. Early nutrition can modulate nervous system development and can lead to epigenetic imprinting. We attempted to increase the sialylated oligosaccharide content of zebrafish yolk reserves, with the aim of evaluating any short-term effects of the treatment on mortality, locomotor behavior, and gene expression. Wild-type embryos were microinjected with saline solution or solutions containing sialylated milk oligosaccharides extracted from human and bovine milk. The results suggest that burst activity and larval survival rates were unaffected by the treatments. Locomotion parameters were found to be similar during the light phase between control and treated larvae; in the dark, however, milk oligosaccharide-treated larvae showed increased test plate exploration. Thigmotaxis results did not reveal significant differences in either the light or the dark conditions. RNA-seq analysis indicated that both treatments exert an antioxidant effect in developing fish. Moreover, sialylated human milk oligosaccharides seemed to increase the expression of genes related to cell cycle control and chromosomal replication, while bovine-derived oligosaccharides caused an increase in the expression of genes involved in synaptogenesis and neuronal signaling. These data shed some light on this poorly explored research field, showing that both human and bovine oligosaccharides support brain proliferation and maturation.

2023-04-04 | GSE218857 | GEO

Chitin oligosaccharide induction

Project description:Strain N16961 grown in M9 + 0.5 % lactate to OD 0.2. Culture was split and one part added 0.6 mM of indicated chitin oligosaccharide. Samples was isolated after 30 and 60 min. cDNA was prepared from 2 ug RNA and labeled with Cy3 (no induction) and Cy5 (induction with chitin oligosaccharide). Samples from at least two independent experiments and a total of four hybridizations for each chitin oligosaccharide and time. A growth condition experiment design type is where some part of the growth condition is changed for the purposes of the experiment, examples of growth conditions changed are media, temperature, humidity, light, nutrients. Keywords: growth_condition_design

2005-08-11 | GSE3101 | GEO

Chitin oligosaccharide induction

2005-08-11 | E-GEOD-3101 | biostudies-arrayexpress

oligosaccharide treatment - 24 hpt-Effect of oligosaccharide treatments on gene expression.

Project description:ra10-03_eliciteur - oligosaccharide treatment - 24 hpt - Do the treatments modify gene expression in a way that can give us a hint as to their use in crop protection? What is the effect of the adjuvant on gene expression? - 6,5-week-old arabidopsis plants grown in short-day photoperiod were spray-treated with water, an adjuvant or oligosaccharides in the adjuvant (10 plants per condition, 4 replicates per condition). 6 hrs, 24 hrs and 48 hrs after the treatment, samples were taken (2 7mm diameter leaf disks per plant, from medium-aged leaves), frozen in liquid nitrogen, and later on total RNA was extracted.

2011-11-01 | GSE30983 | GEO

Transcriptome changes in bovine oocytes caused by microinjection

Project description:Purpose: In this study, we investigated the effect of microinjection on oocytes transcriptome changes through scrambled siRNA injection. Method: Scrambled siRNA injection followed by RNA-seq of single oocytes. Result:Our result showed 119 transcripts were differentially expressed of which 76 were down-regulated after scrambled siRNA injection.

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To establish specific structural evidence that glycans are indeed blunted/altered from the glycan profiling of glycan moieties on the surface of leukocytes

Project description:We want to characterize the glycan moieties on the surface of leukocytes treated with fluorinated analogs of glucosamine. Since these metabolic inhibitors compete with natural-occuring glucosamine used in oligosaccharide chain formation, these compounds have been shown to be effective in lowering Type 2 lactosamine and sialylated Lewis antigens, which results in altered selectin ligand functions.

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Nigral dopaminergic sirtuin 3 inhibits aging-related locomotor decline

Project description:Aging reduces locomotor capacity. Although a decrease in the activity of the nigrostriatal dopamine (DA) system is one of key mechanisms linked to the drop-in locomotor activity in aging, the specific molecule responsible for controlling this decline remains elusive. Here we report that sirtuin 3 (SIRT3), a mitochondrial deacetylase associated with an anti-aging effect, is downregulated in nigral DA neurons of 20-month-old mice, showing the decline in locomotor activity, and that the declined locomotor activity by aging was deteriorated with more serious alteration in the levels of mitochondria-related proteins in mice with genetic deletion of SIRT3 compared to age-matched control mice. Moreover, SIRT3 upregulation through the administration of adeno-associated virus serotype 1 (AAV1) encoding the SIRT3 gene in the substantia nigra (SN) mitigated the age-dependent loss of locomotor activity by the preservation of the nigrostriatal DA system from aging in vivo. Therefore, we conclude that SIRT3 preservation in the nigrostriatal DA system has resistance to age-related locomotor decline, suggesting that SIRT3 upregulation in nigral DA neurons can be useful to preserve locomotor capacity in aging.

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Transcriptome changes in bovine oocytes caused by microinjection

Project description:Transcriptome changes in bovine oocytes caused by microinjection

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Chitin oligosaccharide-responsive genes in suspension-cultured rice cells

Project description:Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. We identified chitine oligosaccharide-responsive genes in suspension-cultured rice cells 1-12 h after treatment using rice 44k microarray. Expression profiling in rice cells treated with chitin oligosaccharide for 1, 2, 4, 6, 8 and 12 h was compared with that in untreated control using two-color method with two biological replicates.

2012-07-24 | E-GEOD-39633 | biostudies-arrayexpress

Cis-regulatory changes in locomotor genes are associated with the evolution of burrowing behavior

Project description:How evolution modifies complex, innate behaviors is largely unknown. Divergence in many morphological traits has been linked, at least in part, to cis-regulatory changes in gene expression, a pattern also observed for some behaviors in recently diverged populations. Given this, we compared the gene expression in the brains of two interfertile sister species of Peromyscus mice, including allele-specific expression (ASE) of their F1 hybrids, that show large and heritable differences in burrowing behavior. Because cis-regulation may contribute to constitutive as well as activity-dependent gene expression, we also captured a molecular signature of burrowing circuit divergence by quantifying gene expression in mice shortly after burrowing. We found that several thousand genes were differentially expressed between the two sister species regardless of behavioral context, with several thousand more showing behavior-dependent differences. Allele-specific expression in F1 hybrids showed a similar pattern, suggesting that much of the differential expression is driven by cis-regulatory divergence. Genes related to locomotor coordination showed the strongest signals of lineage-specific selection on burrowing-induced cis-regulatory changes. By comparing these candidate genes to independent quantitative trait locus (QTL) mapping data, we found that the closest QTL markers to these candidate genes are associated with variation in burrow shape, demonstrating an enrichment for candidate locomotor genes near segregating causal loci. Together, our results provide insight into how cis-regulated gene expression can depend on behavioral context as well as how this dynamic regulatory divergence between species can be integrated with forward genetics to enrich our understanding of the genetic basis of behavioral evolution.

2022-01-12 | GSE193266 | GEO

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