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Circular RNA cPLOD2 regulates pericyte function by targeting KLF4

ABSTRACT: Circular RNA cPLOD2 regulates pericyte function by targeting KLF4

PROVIDER: PRJNA907784 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR22502077.fastq.gz	Fastqsanger.gz
	SRR22502078.fastq.gz	Fastqsanger.gz
	SRR22502079.fastq.gz	Fastqsanger.gz
	SRR22502080.fastq.gz	Fastqsanger.gz
	SRR22502081.fastq.gz	Fastqsanger.gz

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Similar Datasets

Circular RNA cPLOD2 regulates pericyte function by targeting KLF4

Project description:Circular RNAs (circRNAs) are generated by back-splicing and control cellular signaling and phenotypes. Pericytes stabilize the capillary structure and play an important role in the formation and maintenance of new blood vessels. Here, we characterized hypoxia-regulated circRNAs in human pericytes and showed that circPLOD2 is induced by hypoxia and regulates pericyte functions. Silencing of circPLOD2 increased pericyte proliferation, endothelial-pericyte interactions and tube formation. Transcriptional profiling of circPLOD2-depleted cells and epigenomic analyses revealed widespread changes in gene expression and identified the regulation of the transcription factor KLF4 as a key effector of these changes. Importantly, overexpression of KLF4 was sufficient to reverse the effects on pericyte proliferation and endothelial-pericyte interactions observed after circPLOD2 depletion. Together, these data reveal a novel function of circPLOD2 in the control of pericyte proliferation and capillary formation and show that circPLOD2-mediated regulation of KLF4 significantly contributes to the transcriptional response to hypoxia.

2023-06-19 | GSE219237 | GEO

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [RNA-Seq]

Project description:Pulmonary vascular development is essential for alveolarization, and disruption of this process contributes to bronchopulmonary dysplasia (BPD) pulmonary pathology. Proper vascular development requires an orchestration of many cell types within the lung. However, the mechanisms by which pericytes support the endothelium in the postnatal lung remain poorly understood. Here, we identify FOXF2 as a critical transcription factor that governs pericyte maturation and function during postnatal lung development and regeneration. FOXF2 expression in pericytes increases postnatally and is selectively downregulated following neonatal hyperoxic injury. Pdgfrb-CreER mediated Foxf2 deletion in pericytes leads to pericyte hyperplasia, impaired migration, reduced expression of angiogenic factors such as ANGPTL4, and exacerbated alveolar simplification in a neonatal murine model of BPD. Transcriptomic and genomic studies demonstrate that FOXF2 maintains chromatin accessibility at pro-angiogenic loci and modulates paracrine signaling essential for endothelial regeneration. Loss of FOXF2 disrupts pericyte–endothelial crosstalk, impairing angiogenesis and alveolar repair during injury. Our study identifies FOXF2 as a central transcriptional regulator of pericyte-driven vascular niche function in the neonatal lung and underscores the pathogenic role of dysfunctional pericytes in BPD.

2025-12-31 | GSE297573 | GEO

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [scRNA-seq]

2025-12-31 | GSE297575 | GEO

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [CUT&Tag]

2025-12-31 | GSE297570 | GEO

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [CUT&Tag]

Project description:FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [CUT&Tag]

| PRJNA1265203 | ENA

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [RNA-Seq]

Project description:FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [RNA-Seq]

| PRJNA1265207 | ENA

FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [scRNA-seq]

Project description:FOXF2 regulates pericyte function required for pericyte-endothelial signaling during neonatal hyperoxic lung injury [scRNA-seq]

| PRJNA1265209 | ENA

Pericyte sGC signaling dictates tumor vessel formation and tumor microenvironment

Project description:Pericytes and endothelial cells (ECs) are building blocks of blood vessels. While the contributions of ECs to tumor angiogenesis and the tumor microenvironment are well established and multiple drugs that targeting ECs have been developed for clinic use to treat cancers, the underlying mechanisms of pericyte in supporting tumor vessel and in shaping tumor microenvironment remains largely unexplored and no pericyte targeting strategies have been approved yet. This study employs targeted deletion of the NO receptor sGC in pericytes and utilizes single-cell RNA sequencing to elucidate its impact on the tumor microenvironment. The results unveil a disruptive effect on EC-pericyte interactions, subsequently impeding Notch-mediated intercellular crosstalk and prompting extensive transcriptomic reprogramming in both cell types. This vascular alteration further relays to neighboring cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) through paracrine signaling, collectively suppressing tumor growth. Importantly, the inhibition of pericyte sGC has limited influence on quiescent vessels but significantly sensitizes angiogenic vessels to anti-angiogenic treatment. In conclusion, this study underscores the vital role of pericytes in governing tumor vessels and the tumor microenvironment, suggesting that targeting pericyte sGC holds promise for enhancing anti-angiogenic therapy.

2024-02-01 | GSE243313 | GEO

Human tumor pericyte protein mass spectrometry

Project description:Proteomics analysis can reveal the differences of protein expression between mural cells (known as pericytes) from normal adjacent tissues (NPC) and tumors (TPC), implying the effectiveness of pericyte to tumor.

2021-08-24 | PXD026963 | Pride

KLF4 knockdown in human keratinocytes

Project description:RNA sequencing (RNAseq) of N/TERT2G keratinocytes transduced with pooled siRNAs targeting KLF4, or non-targeting control siRNA (siCon)

2020-02-24 | GSE137410 | GEO

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